Tsuchido T, Takano M
Department of Fermentation Technology, Osaka University, Japan.
Antimicrob Agents Chemother. 1988 Nov;32(11):1680-3. doi: 10.1128/AAC.32.11.1680.
The sensitivities of intact and heat-injured cells of Escherichia coli K-12 to several antibacterial compounds were measured by the prolongation of growth delay. Cells exposed to sublethal heat became more sensitive to various hydrophobic compounds, such as medium-chain fatty acids, alkyl esters of p-hydroxybenzoic acid, and some kinds of antibiotics or dyes, than unheated cells; but there was a smaller or no increase in sensitivity to short-chain fatty acids, chloramphenicol, and vancomycin. The destruction by heat of a permeability barrier of the outer membrane may have sensitized the cells to hydrophobic compounds. The sensitization was much lower for a strain defective in lipopolysaccharide, which is important as a barrier against hydrophobic compounds.
通过生长延迟的延长来测定大肠杆菌K-12完整细胞和热损伤细胞对几种抗菌化合物的敏感性。与未加热的细胞相比，暴露于亚致死热的细胞对各种疏水化合物（如中链脂肪酸、对羟基苯甲酸烷基酯以及某些抗生素或染料）更为敏感；但对短链脂肪酸、氯霉素和万古霉素的敏感性增加较小或没有增加。外膜通透性屏障被热破坏可能使细胞对疏水化合物敏感。对于脂多糖有缺陷的菌株，其致敏性要低得多，脂多糖作为抵御疏水化合物的屏障很重要。
