Gao Chunxia, Zhao Ke, Lin Liwei, Wang Jinyu, Liu Yang, Zhu Peizhi
School of Chemistry and Chemical Engineering, Yangzhou University, Jiangsu 225009, China.
Nanomaterials (Basel). 2019 Feb 11;9(2):241. doi: 10.3390/nano9020241.
Hydroxyapatite (HA), a typical inorganic component of bone, is a widely utilized biomaterial for bone tissue repair and regeneration due to its excellent properties. Inspired by the recent findings on the important roles of protein in biomineralization and natural structure of fish scales, keratin was chosen as a template for modulating the assembly of HA nanocrystals. A series of HA nanocrystals with different sizes were synthesized by adjusting the concentration of partially hydrolyzed keratin. The structure and compositions of the prepared HA were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), Raman spectrum, and Transmission electron microscopy (TEM). Results revealed that the size of the synthesized HA nanocrystals can be controlled by adjusting the concentration of partially hydrolyzed keratin. Specifically, the size of synthesized HA decreased from 63 ± 1.5 nm to 27 ± 0.9 nm with the increasing concentration of partially hydrolyzed keratin from 0 to 0.6g. In addition, cytocompatibility of synthesized HA nanocrystals were evaluated using the MG-63 cells.
羟基磷灰石（HA）是骨骼的一种典型无机成分，因其优异的性能而成为广泛用于骨组织修复和再生的生物材料。受近期关于蛋白质在生物矿化和鱼鳞天然结构中重要作用的研究结果启发，角蛋白被选作调节HA纳米晶体组装的模板。通过调节部分水解角蛋白的浓度，合成了一系列不同尺寸的HA纳米晶体。采用傅里叶变换红外（FTIR）光谱、X射线衍射（XRD）、拉曼光谱和透射电子显微镜（TEM）对制备的HA的结构和组成进行了表征。结果表明，通过调节部分水解角蛋白的浓度可以控制合成的HA纳米晶体的尺寸。具体而言，随着部分水解角蛋白浓度从0增加到0.6g，合成的HA尺寸从63±1.5nm减小到27±0.9nm。此外，使用MG-63细胞评估了合成的HA纳米晶体的细胞相容性。
Zotero 插件