农杆菌介导的基因转移对大蒜（Allium sativum L.）的转化与再生
Transformation and regeneration of garlic (Allium sativum L.) by Agrobacterium-mediated gene transfer.
作者信息
Kondo T, Hasegawa H, Suzuki M
机构信息
Aomori Green BioCenter, 221-10, Yamaguchi, Nogi, Aomori, 030-0142, Japan e-mail:
出版信息
Plant Cell Rep. 2000 Oct;19(10):989-993. doi: 10.1007/s002990000222.
By using highly regenerative calluses, we developed a stable transformation system in garlic (Allium sativum L.). The temperature and number of days of co-cultivation with Agrobacterium tumefaciens was shown to be an important factor in transient expression of the uid A gene. After a culture period of 5 months in selection medium containing hygromycin, 20 shoots were induced from ca. 1000 calluses, among which 15 plants expressed β-glucuronidase activity upon staining with X-Gluc. Shoots developed into transgenic garlic after 1 month. Integration of the uid A gene was confirmed by Southern blot analysis for genomic DNA of transgenic garlic plants.
通过使用高度再生的愈伤组织，我们在大蒜（Allium sativum L.）中建立了一个稳定的转化系统。结果表明，与根癌农杆菌共培养的温度和天数是uid A基因瞬时表达的重要因素。在含有潮霉素的选择培养基中培养5个月后，约1000个愈伤组织诱导出20株芽苗，其中15株在用X-Gluc染色后表现出β-葡萄糖醛酸酶活性。1个月后，芽苗发育成转基因大蒜。通过对转基因大蒜植株基因组DNA的Southern杂交分析，证实了uid A基因的整合。
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