Department of Pharmacology & Toxicology, Faculty of Pharmacy, Qassim University, Saudi Arabia.
Department of Pharmacology & Toxicology, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
Oxid Med Cell Longev. 2019 Jan 13;2019:5162687. doi: 10.1155/2019/5162687. eCollection 2019.
Interest is growing in finding natural sources of effective antitumor agents that generate fewer side effects than conventional chemotherapeutic drugs. Avenanthramides (Avns) are such compounds; these phenolic molecules naturally occur in oats and have antioxidant, anti-inflammatory, and antiproliferative effects making them worthy of further research. The aim of this study is to characterise Avns' curative ability and antineoplastic activity on solid-form Ehrlich tumors. For the study, 75 female mice were randomly and equally allocated to five groups (group 1-control, group 2-DMSO, group 3-positive control receiving Avns, group 4-mice with Ehrlich solid tumor, and group 5-Ehrlich solid tumor treated with Avns). Mice with Ehrlich solid tumors exhibit increased tumor volume; elevated expression of AFP, ALT, AST, Bcl2, CEA, cholesterol, creatinine, urea, MDA, PCNA, potassium, triglycerides, TNF-, and NF-B; and a concomitant decline in catalase, GSH, P53, and SOD. In the mice with Ehrlich tumors who received Avns, there appeared to be improvement in NF-B TNF-, tumor markers (AFP and CEA), electrolytes, liver and kidney function enzymes, and lipid profiles; reduced MDA level; improved antioxidant parameters; normalised liver protein, P53, and PCNA; and reduced Bcl2 expression. Pathological examination of tumor lesions also indicated improvement. These results suggest that Avns exhibit antineoplastic activity and possess antioxidant properties that enhance the antioxidant defence system, thus reducing the oxidative stress caused by Ehrlich solid tumors.
人们越来越感兴趣的是寻找比传统化疗药物副作用更少的有效抗肿瘤天然来源。燕麦蒽酰胺(Avns)就是这样的化合物;这些酚类分子天然存在于燕麦中,具有抗氧化、抗炎和抗增殖作用,值得进一步研究。本研究的目的是研究 Avns 对实体型 Ehrlich 肿瘤的治疗能力和抗肿瘤活性。在这项研究中,75 只雌性小鼠被随机平均分配到五组(第 1 组-对照组、第 2 组-DMSO 组、第 3 组接受 Avns 的阳性对照组、第 4 组-Ehrlich 实体瘤小鼠组和第 5 组-Ehrlich 实体瘤用 Avns 治疗组)。患有 Ehrlich 实体瘤的小鼠表现出肿瘤体积增加;甲胎蛋白(AFP)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、B 细胞淋巴瘤-2(Bcl2)、癌胚抗原(CEA)、胆固醇、肌酐、尿素、丙二醛(MDA)、增殖细胞核抗原(PCNA)、钾、甘油三酯、肿瘤坏死因子-α(TNF-α)和核因子-κB(NF-κB)的表达升高;同时过氧化氢酶(CAT)、谷胱甘肽(GSH)、P53 和超氧化物歧化酶(SOD)下降。在接受 Avns 的 Ehrlich 肿瘤小鼠中,NF-κB、TNF-α、肿瘤标志物(AFP 和 CEA)、电解质、肝肾功能酶和脂质谱似乎有所改善;MDA 水平降低;抗氧化参数改善;肝蛋白、P53 和 PCNA 正常化;Bcl2 表达减少。肿瘤病变的组织病理学检查也表明有改善。这些结果表明,Avns 具有抗肿瘤活性,并具有抗氧化特性,可增强抗氧化防御系统,从而减少 Ehrlich 实体瘤引起的氧化应激。