Wang Xin, Mo Fong-Ming, Bo Hao, Xiao Lei, Chen Guan-Yang, Zeng Peng-Wei, Huang Yan-Ning, Lei Zhuo, Yuan Wei-Jie, Chen Zi-Hua
Department of General Surgery, Xiangya Hospital, Central South University, Changsha, China.
Key Laboratory of Nanobiological Technology of National Health and Family Planning Commission, Xiangya Hospital , Central South University, Changsha, China.
J Cancer. 2018 Jul 16;9(16):2834-2843. doi: 10.7150/jca.26046. eCollection 2018.
Through bioinformatics analysis, a novel lncRNA, LINC00460, was implicated in the development of multiple cancers. However, the precise expression pattern, clinical significance and biological function of LINC00460 in colorectal cancer (CRC) remain unknown. Network databases were used to investigate the correlation between LINC00460 and CRC. In situ hybridization was performed to verify the precise expression pattern and clinical significance of LINC00460 in a CRC tissue microarray, which included 92 pairs of CRC and adjacent normal tissues. The effect of LINC00460 on proliferation was evaluated by MTT, colony formation assays and flow cytometry employing SW620 and HCT116 cell lines. Cell migration and matrigel invasion assays were performed to investigate whether LINC00460 is involved in the metastasis of CRC. The expression of LINC00460 was significantly upregulated in CRC tissues and cells, associated with early stage CRC and low disease-free survival. The downregulated of LINC00460 expression increased cell proliferation by regulating the cell cycles of SW620 and HCT116 cells. LINC00460 knockdown did not affect cell migration or invasion in vitro. These findings suggest that LINC00460 may be an interesting target for the development of CRC.
通过生物信息学分析，一种新型长链非编码RNA（lncRNA）LINC00460被认为与多种癌症的发生发展有关。然而，LINC00460在结直肠癌（CRC）中的精确表达模式、临床意义和生物学功能仍不清楚。利用网络数据库研究LINC00460与CRC之间的相关性。采用原位杂交技术在包含92对CRC组织和癌旁正常组织的CRC组织芯片中验证LINC00460的精确表达模式和临床意义。采用MTT法、集落形成试验和流式细胞术，利用SW620和HCT116细胞系评估LINC00460对细胞增殖的影响。进行细胞迁移和基质胶侵袭试验，以研究LINC00460是否参与CRC的转移。LINC00460在CRC组织和细胞中表达显著上调，与早期CRC和低无病生存率相关。下调LINC00460表达可通过调节SW620和HCT116细胞的细胞周期来增加细胞增殖。敲低LINC00460对体外细胞迁移或侵袭无影响。这些发现表明，LINC00460可能是CRC治疗开发中一个有价值的靶点。
