木樨草素通过干扰外壳蛋白 I 复合物的表达来降低甲型流感病毒在体外的产量。
Luteolin decreases the yield of influenza A virus in vitro by interfering with the coat protein I complex expression.
机构信息
Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China.
Key Laboratory of Molecular Imaging of Shanghai Education Commission, Shanghai University of Medicine & Health Sciences, Shanghai, China.
出版信息
J Nat Med. 2019 Jun;73(3):487-496. doi: 10.1007/s11418-019-01287-7. Epub 2019 Feb 13.
Influenza is an acute transmissible respiratory infectious disease in humans and animals with high morbidity and mortality. It was reported that luteolin, extracted from Chinese herbs, could potently inhibit influenza virus replication in vitro. To assess the effect and explore the fundamental mechanism of luteolin, we infected several cell lines with two subtypes of influenza A virus (IAV), including A/Jiangxi/312/2006 (H3N2) and A/Fort Monmouth/1/1947 (H1N1) and demonstrated that luteolin suppressed the replication of IAV by cytopathic effect reduction method, qRT-PCR, immunofluorescence and Western blot assays. A time-of-addition assay indicated that this compound interfered with viral replication at the early stage of infection. In addition, we found that luteolin suppressed coat protein I complex expression, which was related to influenza virus entry and endocytic pathway. Overall, our findings demonstrated the antiviral effect of luteolin against IAV and its novel antiviral mechanism.
流感是一种在人类和动物中具有高发病率和死亡率的急性传染性呼吸道疾病。据报道，从中药中提取的木犀草素可以有效地抑制流感病毒在体外的复制。为了评估木犀草素的作用并探索其基本机制，我们用两种亚型的流感病毒（IAV）感染了几种细胞系，包括 A/Jiangxi/312/2006（H3N2）和 A/Fort Monmouth/1/1947（H1N1），并通过细胞病变效应减少法、qRT-PCR、免疫荧光和 Western blot 检测证实木犀草素抑制了 IAV 的复制。加药时间测定表明，该化合物在感染早期干扰病毒复制。此外，我们发现木犀草素抑制了外壳蛋白 I 复合物的表达，这与流感病毒的进入和内吞途径有关。总的来说，我们的研究结果表明木犀草素具有抗 IAV 的抗病毒作用及其新的抗病毒机制。
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