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生物炭单独及与细菌混合菌剂添加对牛粪堆肥过程中细菌群落改善的积极影响。

Positive impact of biochar alone and combined with bacterial consortium amendment on improvement of bacterial community during cow manure composting.

机构信息

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, China.

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, China.

出版信息

Bioresour Technol. 2019 May;280:79-87. doi: 10.1016/j.biortech.2019.02.026. Epub 2019 Feb 5.

Abstract

The present work studied to evaluate the effectiveness under the interaction of bacterial consortium and biochar applied to give impetus to bacterial community activities among cow manure composting. High-throughput sequencing technique and six treatments were carried out: T2, T3 and T6 were single apply of bacteria culture (C), 12%wood biochar (12%WB) and 12%wheat straw biochar (12%WSB), respectively, while T4 and T5 were bacterial consortium combined with 12%WB and 12%WSB respectively, and T1 was used as control. The conclusion shows that the richness of bacterial community were most prosperity in T5 and T4 that in line with the statistical analysis angle of curves and cluster. The dominate phyla of Firmicutes, Proteobacteria and Bacteroidetes were accounted to 31.36%, 34.79% and 33.85%, the superior genera were Dysgonomonas (16.55%), Empedobater (9.39%), Atopostipes (13.42%), Tissierella (8.25%), Marinimicrobium (14.45%) and Pseudomonas (9%). Overall, bacterial consortium combined with biochar could stimulate microbe activity to accelerate degradation, enhance richness and alter specific selection of bacterial community.

摘要

本研究旨在评估细菌生物群落与生物炭相互作用下对牛粪堆肥中细菌群落活性的促进效果。采用高通量测序技术和 6 种处理方法:T2、T3 和 T6 分别为单一添加细菌培养物(C)、12%木生物炭(12%WB)和 12%小麦秸秆生物炭(12%WSB),而 T4 和 T5 分别为细菌生物群落与 12%WB 和 12%WSB 结合使用,T1 作为对照。结论表明,T5 和 T4 中的细菌群落丰富度最为繁荣,这与曲线和聚类的统计分析角度一致。厚壁菌门、变形菌门和拟杆菌门是主要的优势菌门,分别占 31.36%、34.79%和 33.85%,优势属为Dysgonomonas(16.55%)、Empedobater(9.39%)、Atopostipes(13.42%)、Tissierella(8.25%)、Marinimicrobium(14.45%)和假单胞菌(9%)。总的来说,细菌生物群落与生物炭的结合可以刺激微生物活性,加速降解,提高丰富度,并改变细菌群落的特定选择。

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