Novel Bacteria and Drug Discovery (NBDD) Research Group, School of Pharmacy, Monash University Malaysia, 47500 Bandar Sunway, Selangor Darul Ehsan, Malaysia.
Biofunctional Molecule Exploratory Research Group (BMEX), School of Pharmacy, Monash University Malaysia, Bandar Sunway, Selangor Darul Ehsan, Malaysia.
Biomed Res Int. 2018 Apr 1;2018:4823126. doi: 10.1155/2018/4823126. eCollection 2018.
Mangrove derived microorganisms constitute a rich bioresource for bioprospecting of bioactive natural products. This study explored the antioxidant potentials of bacteria derived from mangrove soil. Based on 16S rRNA phylogenetic analysis, strain MUM292 was identified as the genus . Strain MUM292 showed the highest 16S rRNA gene sequence similarity of 99.54% with NBRC12873. Furthermore, strain MUM292 was also characterized and showed phenotypic characteristics consistent with bacteria. Fermentation and extraction were performed to obtain the MUM292 extract containing the secondary metabolites of strain MUM292. The extract displayed promising antioxidant activities, including DPPH, ABTS, and superoxide radical scavenging and also metal-chelating activities. The process of lipid peroxidation in lipid-rich product was also retarded by MUM292 extract and resulted in reduced MDA production. The potential bioactive constituents of MUM292 extract were investigated using GC-MS and preliminary detection showed the presence of pyrazine, pyrrole, cyclic dipeptides, and phenolic compound in MUM292 extract. This work demonstrates that MUM292 can be a potential antioxidant resource for food and pharmaceutical industries.
红树林来源的微生物构成了生物活性天然产物生物勘探的丰富生物资源。本研究探索了红树林土壤来源细菌的抗氧化潜力。基于 16S rRNA 系统发育分析，鉴定菌株 MUM292 为 属。菌株 MUM292 与 NBRC12873 的 16S rRNA 基因序列相似性最高，为 99.54%。此外，菌株 MUM292 还具有与 细菌一致的表型特征。进行发酵和提取以获得含有菌株 MUM292 次生代谢产物的 MUM292 提取物。提取物表现出有希望的抗氧化活性，包括 DPPH、ABTS 和超氧自由基清除活性以及金属螯合活性。MUM292 提取物还能延缓富含脂质的产品中脂质过氧化过程，减少 MDA 的产生。使用 GC-MS 研究了 MUM292 提取物的潜在生物活性成分，初步检测表明 MUM292 提取物中存在吡嗪、吡咯、环二肽和酚类化合物。这项工作表明 MUM292 可以成为食品和制药工业的潜在抗氧化资源。
