Biofunctional Molecule Exploratory Research Group (BMEX), School of Pharmacy, Monash University Malaysia, 47500 Bandar Sunway, Subang Jaya, Selangor Darul Ehsan, Malaysia.
Novel Bacteria and Drug Discovery Research Group (NBDD), Microbiome and Bioresource Research Strength (MBRS), Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, 47500 Bandar Sunway, Subang Jaya, Selangor Darul Ehsan, Malaysia.
Biomed Res Int. 2020 Mar 11;2020:6402607. doi: 10.1155/2020/6402607. eCollection 2020.
The mangrove ecosystem of Malaysia remains yet to be fully explored for potential microbes that produce biologically active metabolites. In the present study, a mangrove-derived sp. strain MUSC 14 previously isolated from the state of Pahang, Malaysia Peninsula, was studied for its potential in producing antioxidant metabolites. The identity of sp. strain MUSC14 was consistent with the genotypic and phenotypic characteristics of the genus. The antioxidant potential of sp. strain MUSC 14 was determined through screening of its methanolic extract against sets of antioxidant assays. The results were indicative of sp. strain MUSC 14 displaying strong antioxidant activity against ABTS, DPPH free radicals and metal chelating activity of 62.71 ± 3.30%, 24.71 ± 2.22%, and 55.82 ± 2.35%, respectively. The result of ferric reducing activity measured in terms of dose was equivalent to 2.35-2.45 g of positive control ascorbic acid. Furthermore, there was a high correlation between the total phenolic content and the antioxidant activities with = 0.979, = 0.858, and = 0.983 representing ABTS, DPPH, and metal chelation, respectively. Overall, the present study suggests that sp. strain MUSC 14 from mangrove forest soil has potential to produce antioxidant metabolites that can be further exploited for therapeutic application.
马来西亚的红树林生态系统尚未被充分探索其产生具有生物活性代谢物的潜在微生物。在本研究中，研究了先前从马来西亚半岛彭亨州分离出的红树林来源的 sp.菌株 MUSC 14，以研究其产生抗氧化代谢物的潜力。sp.菌株 MUSC14 的身份与属的基因型和表型特征一致。通过对其甲醇提取物进行一系列抗氧化测定来确定 sp.菌株 MUSC 14 的抗氧化潜力。结果表明，sp.菌株 MUSC 14 对 ABTS、DPPH 自由基和金属螯合活性显示出强烈的抗氧化活性，分别为 62.71 ± 3.30%、24.71 ± 2.22%和 55.82 ± 2.35%。以剂量表示的铁还原活性的结果相当于 2.35-2.45 g 的阳性对照抗坏血酸。此外，总酚含量与抗氧化活性之间存在高度相关性，分别为 = 0.979、 = 0.858 和 = 0.983，代表 ABTS、DPPH 和金属螯合。总的来说，本研究表明，来自红树林土壤的 sp.菌株 MUSC 14 具有产生抗氧化代谢物的潜力，可进一步开发用于治疗应用。
