Wang Wei, Sun Wenchao, Cao Liang, Zheng Min, Zhu Yilong, Li Wenjie, Liu Cunxia, Zhuang Xinyu, Xing Jialiang, Lu Huijun, Luo Tingrong, Jin Ningyi
College of Animal Science and Technology, Guangxi University, Nanning, 530004, China.
Institute of Military Veterinary Medicine, Academy of Military Sciences, Changchun, 130122, China.
BMC Vet Res. 2019 Feb 13;15(1):60. doi: 10.1186/s12917-019-1793-0.
Porcine circovirus type 3 (PCV3) is a single-stranded, closed circular DNA virus, which causes porcine dermatitis and nephropathy syndrome (PDNS), multisystemic inflammation, and reproductive failure. The present study aimed to investigate the seroprevalence of PCV3 in cattle (Bos taurus) in Shandong province, China, and examine its genome diversity.
PCR amplification and sequencing showed that 74 of 213 bovine samples (34.7%) were positive for PCV3. Among them, the capsid gene (n = 12) and the complete genome (n = 4) were sequenced. These sequences had high identities to the reference capsid gene (98.0-100%) and the complete genome (97.5-99.8%). The PCV3 strains were classified into two different genotypes (PCV3a and PCV3b), according to phylogenetic analysis based on the complete genome and capsid gene sequences. Specifically, the bovine-origin strains in this study were grouped into PCV3a, showing a close relationship with PCV3-US/SD2016 (American strain; GenBank: KX966193.1). Notably, a comparison of the inferred amino acid sequences revealed a mutation from D124 to Y124.
This was the first seroprevalence and genetic investigation of PCV3 in cattle in Shandong province, China. The results could provide insights into the epidemiology and pathogenesis of this important virus.
猪圆环病毒3型（PCV3）是一种单链闭合环状DNA病毒，可引起猪皮炎肾病综合征（PDNS）、多系统炎症和繁殖障碍。本研究旨在调查中国山东省牛（Bos taurus）群中PCV3的血清流行率，并检测其基因组多样性。
PCR扩增和测序显示，213份牛样本中有74份（34.7%）PCV3呈阳性。其中，对衣壳基因（n = 12）和全基因组（n = 4）进行了测序。这些序列与参考衣壳基因（98.0 - 100%）和全基因组（97.5 - 99.8%）具有高度同源性。根据基于全基因组和衣壳基因序列的系统发育分析，PCV3毒株被分为两种不同的基因型（PCV3a和PCV3b）。具体而言，本研究中源自牛的毒株被归为PCV3a，与PCV3-US/SD2016（美国毒株；GenBank: KX966193.1）关系密切。值得注意的是，对推导的氨基酸序列进行比较发现有一个从D124到Y124的突变。
这是中国山东省首次对牛群进行PCV3血清流行率和遗传学调查。研究结果可为深入了解这种重要病毒的流行病学和发病机制提供依据。
