Institute of Virology, Wenzhou University, Wenzhou, China; Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, China.
College of Animal Science and Technology, Guangxi University, Nanning, China; Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, China.
Virus Res. 2019 Sep;270:197663. doi: 10.1016/j.virusres.2019.197663. Epub 2019 Jul 10.
Porcine circovirus type 3 (PCV3) is an emerging circovirus species associated with several diseases. The study aimed to investigate the frequency of porcine circovirus 3 (PCV3) and its coinfection with canine parvovirus type 2 (CPV-2) in dogs in the Guangxi province from 2015 to 2017, China, and to examine the genome diversity of PCV3. Using polymerase chain reaction (PCR) amplification and sequencing, 96 of 406 (23.6%)samples were positive for PCV3, 38 out of 406 (9.4%) samples were coinfected with both PCV3 and CPV-2. The CPV-positive rate was significantly higher in the PCV3-positive samples than in the non-PCV3 samples, and the difference was extremely significant (P < 0.01). The complete genome (n=4) and ten capsid genes (n=10) of PCV3 were sequenced. Multiple sequence alignment results showed that these sequences shared 98.5-100% nucleotide similarity with the reference genome sequence and 97.5-100% nucleotide similarity with the reference capsid gene sequence. PCV3 was classified into two different genotypes, according to phylogenetic analysis based on the whole genome. These strains were clustered in PCV3a, showing a close relationship with PCV3-US/SD2016. Surprisingly, we separately analyzed these PCV3 strains from the Guangxi province and found that the dog and pig PCV3 are from different branches. In summary, this was the first seroprevalence and genetic investigation of PCV3 in dogs in the Guangxi province, China, and the first complete genome PCV3 from dogs obtained in the world. The results provide insights into the epidemiology and pathogenesis of this important virus.
猪圆环病毒 3 型（PCV3）是一种新兴的圆环病毒属病毒，与多种疾病有关。本研究旨在调查 2015 年至 2017 年期间中国广西省犬中猪圆环病毒 3 型（PCV3）及其与犬细小病毒 2 型（CPV-2）的混合感染情况，并检测 PCV3 的基因组多样性。通过聚合酶链反应（PCR）扩增和测序，在 406 份样本中，96 份（23.6%）为 PCV3 阳性，38 份（9.4%）为 PCV3 和 CPV-2 混合感染。PCV3 阳性样本中 CPV 的阳性率明显高于非 PCV3 样本，差异极显著（P<0.01）。对 4 株 PCV3 全基因组和 10 株衣壳基因进行了测序。多序列比对结果显示，这些序列与参考基因组序列的核苷酸相似性为 98.5-100%，与参考衣壳基因序列的核苷酸相似性为 97.5-100%。基于全基因组的系统进化分析将 PCV3 分为两种不同的基因型。这些株分为 PCV3a 型，与 PCV3-US/SD2016 密切相关。令人惊讶的是，我们分别分析了来自广西省的这些 PCV3 株，发现犬和猪的 PCV3 来自不同的分支。总之，这是中国广西省首次对犬 PCV3 的血清流行率和遗传进行调查，也是世界上首次获得犬的 PCV3 完整基因组。研究结果为了解该重要病毒的流行病学和发病机制提供了依据。
