Research Institute of Donkey High-efficiency Breeding and Ecological Feeding, College of Agronomy, Liaocheng University, Liaocheng, China.
Delisi Group Co., LTD, Zhucheng, China.
BMC Vet Res. 2021 Sep 18;17(1):308. doi: 10.1186/s12917-021-03013-6.
PCV3 is a pathogen associated with porcine dermatitis and nephropathy syndrome (PDNS)-like clinical signs, reproductive failure, and cardiac and multiorgan inflammation, which was newly identified in 2016 in sows in USA. Recently, PCV3 has also been identified from several non-porcine species like (cattle, dog, wild boar, deer, mice and ticks). However, PCV3 infection in donkey is not well established. Since 2019, 300 blood samples were collected from female donkey, which was characterized by abortion and sterility, in Liaocheng city of China.
In the present study, an investigation of PCV3 in donkey blood samples was undertaken employing by real time PCR. Positive rates of PCV3 in donkeys reach to 21.0 %. In addition, one full-length PCV3 genome sequence was obtained, and it had a highest identity with porcine circovirus 3 PCV3/CN/Nanjing2017 strain and is clustered to PCV3a genotype based on ORF2 sequences.
This is the first report of detection of PCV3 from female donkeys presenting reproductive failure in large-scale donkey farms, China. In addition, the PCV3 strain identified in this study shared the closest relationship with those from porcine, suggesting that PCV3 may be transmitted from pigs to donkeys. Totally, PCV3 infection in donkey should be concerned although the association between it and reproductive failure are not better understood.
PCV3 是一种与猪皮炎肾病综合征（PDNS）样临床症状、繁殖失败以及心脏和多器官炎症相关的病原体，于 2016 年在美国母猪中首次被发现。最近，PCV3 也已从几种非猪物种（牛、狗、野猪、鹿、鼠和蜱）中被鉴定出来。然而，驴的 PCV3 感染尚未得到充分证实。自 2019 年以来，从中国聊城市表现为流产和不育的雌性驴中采集了 300 份血液样本。
本研究采用实时 PCR 对驴血液样本中的 PCV3 进行了调查。驴中 PCV3 的阳性率达到 21.0%。此外，还获得了一个全长 PCV3 基因组序列，其与猪圆环病毒 3 PCV3/CN/Nanjing2017 株的同源性最高，根据 ORF2 序列聚类为 PCV3a 基因型。
这是首次在中国大型驴场的表现繁殖失败的雌性驴中检测到 PCV3 的报告。此外，本研究中鉴定的 PCV3 株与猪源 PCV3 株的亲缘关系最近，表明 PCV3 可能从猪传播给驴。尽管 PCV3 感染与繁殖失败之间的关系尚未得到更好的理解，但驴的 PCV3 感染仍应引起关注。
