Gajardo A, Fiore N, Prodan S, Paltrinieri S, Botti S, Pino A M, Zamorano A, Montealegre J, Bertaccini A
Facultad de Ciencias Agronómicas-Universidad de Chile, Santiago, Chile.
Dipartimento di Scienze e Tecnologie Agroambientali, Patologia Vegetale, Alma Mater Studiorum-Universitá di Bologna, Bologna, Italy.
Plant Dis. 2009 Aug;93(8):789-796. doi: 10.1094/PDIS-93-8-0789.
An extensive survey was performed from 2002 to 2006 to detect and identify phytoplasmas associated with Chilean grapevines. Nested polymerase chain reaction assays using phytoplasma universal primer pairs P1/P7 and R16F2n/R2 detected phytoplasmas in 34 out of the 94 samples tested (36%). Restriction fragment length polymorphism (RFLP) analyses, cloning, and sequencing allowed identification of phytoplasmas belonging to ribosomal subgroups 16SrI-B, 16SrI-C, 16SrVII-A, and 16SrXII-A. The 16SrVII-A phytoplasma represents a new finding in grapevine; moreover, variability of the RFLP profile was observed in some of the 16SrXII-A phytoplasmas, indicating possible new ribosomal subgroups. Mixed phytoplasma infections and infections of phytoplasmas together with one or more viruses also occurred.
2002年至2006年期间进行了一项广泛的调查，以检测和鉴定与智利葡萄藤相关的植原体。使用植原体通用引物对P1/P7和R16F2n/R2的巢式聚合酶链反应分析在94个测试样本中的34个（36%）中检测到了植原体。限制性片段长度多态性（RFLP）分析、克隆和测序使得能够鉴定出属于核糖体亚组16SrI-B、16SrI-C、16SrVII-A和16SrXII-A的植原体。16SrVII-A植原体是葡萄藤中的一项新发现；此外，在一些16SrXII-A植原体中观察到RFLP图谱的变异性，表明可能存在新的核糖体亚组。还发生了混合植原体感染以及植原体与一种或多种病毒的共同感染。
