Katas Haliza, Lim Chei Sin, Nor Azlan Ahmad Yasser Hamdi, Buang Fhataheya, Mh Busra Mohd Fauzi
Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.
Tissue Engineering Centre, UKM Medical Centre, 56000, Cheras, Kuala Lumpur, Malaysia.
Saudi Pharm J. 2019 Feb;27(2):283-292. doi: 10.1016/j.jsps.2018.11.010. Epub 2018 Nov 23.
A simple, cost-effective, and environmentally friendly method is needed for synthesizing metal nanoparticles, including gold nanoparticles (AuNPs). In this study, AuNPs were synthesized with sclerotial extract (LRE) and chitosan (CS) as reducing and stabilizing agents, respectively. Different LRE concentrations from cold and hot water extraction (CWE and HWE, respectively) were used to reduce chloroauric acid (HAuCl) to form AuNPs. Positively charged chitosan stabilized AuNPs (CS-AuNPs) mediated by LRE exhibited a surface plasmon resonance (SPR) band at 533 nm. The CS-AuNPs synthesized using CWE had a smaller particle size (49.5 ± 6.7-82.4 ± 28.0 nm) compared to that of the HWE samples (80.3 ± 23.4-125.3 ± 41.5 nm), depending on LRE concentration. FTIR results suggested protein and polysaccharides in LRE were the sources of reducing power, reducing gold ions to AuNPs. CS-AuNPs were mostly spherical with higher LRE concentrations, whereas some triangular, pentagonal, irregular, and rod shaped AuNPs were observed at lower LRE concentrations. CS-AuNPs mediated by LRE displayed effective antibacterial activity against gram-negative ( and ) and gram-positive bacteria ( and sp.). Thus, the biosynthesized AuNPs using LRE and chitosan provide opportunities for developing stable and eco-friendly nanoparticles with effective antibacterial properties.
需要一种简单、经济高效且环保的方法来合成金属纳米颗粒，包括金纳米颗粒（AuNPs）。在本研究中，分别以菌核提取物（LRE）和壳聚糖（CS）作为还原剂和稳定剂来合成AuNPs。使用来自冷水和热水提取（分别为CWE和HWE）的不同LRE浓度将氯金酸（HAuCl）还原以形成AuNPs。由LRE介导的带正电荷的壳聚糖稳定的AuNPs（CS-AuNPs）在533nm处表现出表面等离子体共振（SPR）带。根据LRE浓度，使用CWE合成的CS-AuNPs的粒径（49.5±6.7 - 82.4±28.0nm）比HWE样品（80.3±23.4 - 125.3±41.5nm）更小。傅里叶变换红外光谱（FTIR）结果表明，LRE中的蛋白质和多糖是还原能力的来源，可将金离子还原为AuNPs。在较高LRE浓度下，CS-AuNPs大多为球形，而在较低LRE浓度下观察到一些三角形、五边形、不规则和棒状的AuNPs。由LRE介导的CS-AuNPs对革兰氏阴性菌（和）和革兰氏阳性菌（和sp.）显示出有效的抗菌活性。因此，使用LRE和壳聚糖生物合成的AuNPs为开发具有有效抗菌性能的稳定且环保的纳米颗粒提供了机会。
