Research Institute of Integrative Life Sciences, Dongguk University-Seoul, Goyangsi 10326, Republic of Korea.
Int J Mol Sci. 2023 May 30;24(11):9477. doi: 10.3390/ijms24119477.
In recent times, numerous natural materials have been used for the fabrication of gold nanoparticles (AuNPs). Natural resources used for the synthesis of AuNPs are more environment friendly than chemical resources. Sericin is a silk protein that is discarded during the degumming process for obtaining silk. The current research used sericin silk protein waste materials as the reducing agent for the manufacture of gold nanoparticles (SGNPs) by a one-pot green synthesis method. Further, the antibacterial effect and antibacterial mechanism of action, tyrosinase inhibition, and photocatalytic degradation potential of these SGNPs were evaluated. The SGNPs displayed positive antibacterial activity (8.45-9.58 mm zone of inhibition at 50 μg/disc) against all six tested foodborne pathogenic bacteria, namely, DB01, ATCC 13565, ATCC 33090, O157:H7 ATCC 23514, ATCC 7966, and ATCC 27583. The SGNPs also exhibited promising tyrosinase inhibition potential, with 32.83% inhibition at 100 μg/mL concentration as compared to 52.4% by Kojic acid, taken as a reference standard compound. The SGNPs also displayed significant photocatalytic degradation effects, with 44.87% methylene blue dye degradation after 5 h of incubation. Moreover, the antibacterial mode of action of the SGNPs was also investigated against and , and the results show that due to the small size of the nanomaterials, they could have adhered to the surface of the bacterial pathogens, and could have released more ions and dispersed in the bacterial cell wall surrounding environment, thereby disrupting the cell membrane and ROS production, and subsequently penetrating the bacterial cells, resulting in lysis or damage to the cell by the process of structural damage to the membrane, oxidative stress, and damage to the DNA and bacterial proteins. The overall outcome of the current investigation concludes the positive effects of the obtained SGNPs and their prospective applications as a natural antibacterial agent in cosmetics, environmental, and foodstuff industries, and for the management of environmental contagion.
近年来，人们已经使用了许多天然材料来制备金纳米粒子（AuNPs）。与化学资源相比，用于合成 AuNPs 的天然资源更加环保。丝胶是一种在获得丝绸时去除的丝蛋白。本研究使用丝胶丝蛋白废料作为还原剂，通过一锅法绿色合成方法制备金纳米粒子（SGNPs）。进一步评估了这些 SGNPs 的抗菌效果和作用机制、酪氨酸酶抑制作用以及光催化降解潜力。SGNPs 对所有六种测试的食源性病原体均表现出积极的抗菌活性（在 50μg/disc 时抑菌圈为 8.45-9.58mm），分别为 DB01、ATCC 13565、ATCC 33090、O157:H7 ATCC 23514、ATCC 7966 和 ATCC 27583。SGNPs 还表现出有希望的酪氨酸酶抑制潜力，在 100μg/mL 浓度下抑制率为 32.83%，而参考标准化合物 Kojic 酸的抑制率为 52.4%。SGNPs 还表现出显著的光催化降解效果，孵育 5 小时后亚甲基蓝染料的降解率为 44.87%。此外，还研究了 SGNPs 对 和 的抗菌作用模式，结果表明，由于纳米材料的尺寸较小，它们可能附着在细菌病原体的表面，并可能释放更多的离子并分散在细菌细胞壁周围的环境中，从而破坏细胞膜和 ROS 的产生，随后穿透细菌细胞，导致细胞膜结构损伤、氧化应激以及 DNA 和细菌蛋白的损伤，从而导致细胞裂解或损伤。当前研究的总体结果得出结论，所获得的 SGNPs 具有积极的效果，并且它们有望作为天然抗菌剂在化妆品、环境和食品工业中得到应用，以及用于管理环境污染。
