Liu Zhiping, Wang Hua, Jiang Hong, Gameiro Giovana Rosa, Wang Jianhua
1Ophthalmic Center, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong China.
2Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, 1638 NW 10th Avenue, McKnight Building - Room 202A, Miami, FL 33136 USA.
Eye Vis (Lond). 2019 Feb 2;6:5. doi: 10.1186/s40662-019-0130-9. eCollection 2019.
The goal was to quantitatively analyze the bulbar conjunctival microvascular density using optical coherence tomography angiography (OCTA) and compare it to the vessel density using functional slit-lamp biomicroscopy (FSLB).
Temporal bulbar conjunctiva of 20 eyes (10 healthy subjects) was imaged using both OCTA and FSLB. Image processing was performed including equalization, de-noising, thresholding, and skeletonization. The vessel density was measured by fractal analysis (box counting, Dbox) and pixel counting (%).
Vessel density (Dbox) of the bulbar conjunctiva obtained using OCTA was 1.28 ± 0.01 Dbox, which was significantly lower than the result (1.32 ± 0.01 Dbox, < 0.001) obtained using FSLB. Furthermore, the vessel density (%) obtained using OCTA was 3.31 ± 0.12%, which was also significantly lower than the result (3.69 ± 0.16%, < 0.001) obtained using FSLB. No significant correlations (r ranged from 0.21 to 0.32, > 0.05) between both instruments were found in both vessel density methods (Dbox and percentage). However, in each of the devices, vessel density in Dbox was significantly correlated with the vessel density in percentage ( = 1.0 for FSLB and = 0.98 for OCTA, both < 0.001).
This study demonstrated that the vessel density of the bulbar conjunctiva obtained using OCTA can be quantified, and the results were not compatible with that obtained using slit-lamp biomicroscopy photography.
目的是使用光学相干断层扫描血管造影（OCTA）对球结膜微血管密度进行定量分析，并将其与使用功能性裂隙灯生物显微镜检查（FSLB）得出的血管密度进行比较。
使用OCTA和FSLB对20只眼（10名健康受试者）的颞侧球结膜进行成像。进行了包括均衡化、去噪、阈值处理和骨架化在内的图像处理。通过分形分析（盒计数，Dbox）和像素计数（%）测量血管密度。
使用OCTA获得的球结膜血管密度（Dbox）为1.28±0.01 Dbox，显著低于使用FSLB获得的结果（1.32±0.01 Dbox，<0.001）。此外，使用OCTA获得的血管密度（%）为3.31±0.12%，也显著低于使用FSLB获得的结果（3.69±0.16%，<0.001）。在两种血管密度测量方法（Dbox和百分比）中，两种仪器之间均未发现显著相关性（r范围为0.21至0.32，>0.05）。然而，在每种设备中，Dbox中的血管密度与百分比中的血管密度显著相关（FSLB为1.0，OCTA为0.98，均<0.001）。
本研究表明，使用OCTA获得的球结膜血管密度可以进行量化，且结果与使用裂隙灯生物显微镜摄影获得的结果不相符。
