Department of Epidemiology and Prevention, Clinical Research Center, National Center for Global Health and Medicine, Tokyo, Japan.
Nutrition. 2013 Oct;29(10):1219-23. doi: 10.1016/j.nut.2013.03.014. Epub 2013 Jun 22.
Higher vitamin B status has been linked to a lower risk for cancer, but the underlying mechanism remains elusive. The aim of the present study was to examine the association of pyridoxal, folate, and homocysteine (Hcy) with urinary 8-hydroxydeoxyguanosine (8-OHdG), a marker of oxidative DNA damage.
The participants were 500 employees (293 men and 207 women), ages 21 to 66 y, of two municipal offices in Japan. Serum pyridoxal and Hcy concentrations were measured using high-performance liquid chromatography (HPLC) method, and serum folate concentrations were measured using chemiluminescent immunoassay. Urinary 8-OHdG concentrations were measured using HPLC method. Multiple regression was used to estimate means of 8-OHdG for each tertile of pyridoxal, folate, and Hcy with adjustment for potential confounders.
In multivariate analysis, 8-OHdG concentration was inversely associated with pyridoxal concentration in men (P for trend = 0.045) but not in women. The association in men was confined to non-smokers (P for trend = 0.033) or those who consumed no or < 20 g/d of ethanol (P for trend = 0.048). 8-OHdG concentrations were not appreciably associated with folate and Hcy concentrations.
The results suggest that vitamin B6, but not folate and homocysteine, plays a role against oxidative DNA damage in Japanese men.
较高的维生素 B 状态与癌症风险降低有关，但潜在机制仍不清楚。本研究旨在研究吡哆醛、叶酸和同型半胱氨酸（Hcy）与尿液 8-羟基脱氧鸟苷（8-OHdG）的关系，8-OHdG 是氧化 DNA 损伤的标志物。
参与者为日本两个市政办公室的 500 名员工（293 名男性和 207 名女性），年龄在 21 至 66 岁之间。使用高效液相色谱法（HPLC）测定血清吡哆醛和 Hcy 浓度，用化学发光免疫分析法测定血清叶酸浓度。使用 HPLC 法测定尿液 8-OHdG 浓度。使用多元回归估计每个吡哆醛、叶酸和 Hcy 三分位组的 8-OHdG 均值，并进行潜在混杂因素的调整。
在多变量分析中，8-OHdG 浓度与男性的吡哆醛浓度呈负相关（趋势 P = 0.045），但与女性无关。这种关联仅局限于不吸烟的男性（趋势 P = 0.033）或不饮酒或饮酒量<20g/d 的男性（趋势 P = 0.048）。8-OHdG 浓度与叶酸和 Hcy 浓度无明显相关性。
结果表明，维生素 B6（而非叶酸和同型半胱氨酸）在日本男性中对氧化 DNA 损伤起作用。
