Pappu H R, Druffel K B, Eastwell K C
Department of Plant Pathology, Washington State University, Pullman 99164-6430.
Washington State University Irrigated Agriculture Research and Extension Center, 24106 N. Bunn Road, Prosser 99350-8694.
Plant Dis. 2008 Jul;92(7):1136. doi: 10.1094/PDIS-92-7-1136C.
Canna (Canna indica) is an important nursery/landscape plant in Washington State with several nurseries producing canna plants for wholesale and retail businesses. Canna plants showing symptoms such as mottling, general yellowing, and veinal chlorosis were found to be widespread (40% symptomatic plants in a nursery of more than 2,000 plants) in Grant County, WA in September 2007. Symptomatic leaves from five plants of each of the following cultivars were tested: Richard Wallace, Crimson Beauty, Wyoming, Petoria, Pink Beauty, Robert Kemp, and Black Knight. Electron microscopic examination of leaf-dip preparations from symptomatic leaves showed badnavirus-like particles of approximately 120 × 30 nm. A badnavirus, Canna yellow mottle virus (CaYMV) (family Caulimoviridae, genus Badnavirus) from canna was first reported from Japan (4) and later in the United States (1,3). Most recently, CaYMV was reported from Italy and the Netherlands (2). Samples were tested for CaYMV by PCR using CaYMV-specific primers, CaYMV-3 (5'- GAC TTC CTG GGT GCA ACA AT -3') and CaYMV-4 (5'- TCT GTG CAA TCT TGG CGT AG -3') (2), which produced a 565-bp amplicon. All samples tested gave the amplicon of expected size. The amplicon from one leaf sample from each of the cultivars was cloned and sequenced. Sequence comparisons with those available in the GenBank confirmed that the sequence obtained was that of CaYMV (95% sequence identity). Increased awareness of the prevalence of CaYMV in nurseries and avoiding the propagation and distribution of infected plants are necessary to minimize the further spread of this virus in canna. References: (1) B. E. L. Lockhart. Acta Hortic. 234:69, 1988. (2) M. T. Marino et al. Online publication. New Disease Reports. http://www.bspp.org.uk/NDR/july2007/2007-08.asp , 2007. (3) M. T. Momol et al. Online publication. doi:10.1094/PHP-2004-0809-01-HN. Plant Health Progress, 2004. (4) S. Yamashita et al. Ann. Phytopathol. Soc. Jpn. 51:642, 1985.
美人蕉（Canna indica）是华盛顿州一种重要的苗圃/园林植物，有几家苗圃为批发和零售企业培育美人蕉植株。2007年9月，在华盛顿州格兰特县发现，表现出斑驳、整体发黄和叶脉黄化等症状的美人蕉植株广泛存在（在一个拥有2000多株植株的苗圃中，40%的植株有症状）。对以下每个品种的5株有症状植株的叶片进行了检测：理查德·华莱士、深红美人、怀俄明、佩托里亚、粉红美人、罗伯特·肯普和黑骑士。对有症状叶片的叶浸制剂进行电子显微镜检查，发现了大小约为120×30纳米的类杆状病毒颗粒。一种来自美人蕉的杆状病毒，美人蕉黄斑驳病毒（CaYMV）（花椰菜花叶病毒科，杆状病毒属）最早在日本被报道（4），后来在美国也有报道（1,3）。最近，意大利和荷兰也报道了CaYMV（2）。使用CaYMV特异性引物CaYMV-3（5'-GAC TTC CTG GGT GCA ACA AT-3'）和CaYMV-4（5'-TCT GTG CAA TCT TGG CGT AG-3'）（2）通过PCR对样品进行CaYMV检测，该引物产生一个565碱基对的扩增子。所有检测的样品都产生了预期大小的扩增子。对每个品种的一个叶片样品的扩增子进行了克隆和测序。与GenBank中可用序列的比对证实，获得的序列是CaYMV的序列（序列同一性为95%）。提高对苗圃中CaYMV流行情况的认识，并避免传播和分发受感染的植株，对于尽量减少这种病毒在美人蕉中的进一步传播是必要的。参考文献：（1）B.E.L.洛克哈特。《园艺学报》234:69，1988。（2）M.T.马里诺等人。在线发表。《新病害报告》。http://www.bspp.org.uk/NDR/july2007/2007-08.asp，2007。（3）M.T.莫莫尔等人。在线发表。doi:10.1094/PHP-2004-0809-01-HN。《植物健康进展》，2004。（4）S.山下等人。《日本植物病理学会年报》51:642，1985。
