Ward L I, Tang J Z, Clover G R G
Plant Health and Environment Laboratory, MAF Biosecurity New Zealand, P.O. Box 2095, Auckland 1140, New Zealand.
Plant Dis. 2008 Jul;92(7):1134. doi: 10.1094/PDIS-92-7-1134B.
Wisteria vein mosaic virus (WVMV) is a member of the Potyvirus genus. The virus has been reported in Wisteria spp. in Australia, China, the United States, and a number of European countries (2). In 2006, several W. sinensis plants with mottling and mosaic symptoms were observed in a commercial plant nursery in Whenuapai, north of Auckland, New Zealand. These plants had been propagated from a nursery in the New Plymouth area of New Zealand. Sap from the symptomatic Wisteria plants was examined with an electron microscope and elongated and flexuous potyvirus-like particles approximately 750 nm long were observed. RNA was extracted from the symptomatic plants with a Qiagen RNeasy Plant Mini Kit (Doncaster, Australia). The RNA was initially tested using general potyvirus primers, PV1/SP6 (4) and U335 (3), with the cycling conditions of 94°C for 5 min followed by 40 cycles of 94°C for 45 s, 50°C for 45 s, 72°C for 90 s, and a final extension of 72°C for 7 min. The polymerase chain reaction (PCR) product (695 bp) was directly sequenced (GenBank Accession No. EU580146) and a BLAST search in GenBank showed 98% nucleotide identity with WVMV (GenBank Accession no. AF484549). The RNA was then tested using WVMV-specific primers, WVMVF1 and WVMVR1, and the published cycling conditions (2). PCR amplicons of 701 bp were obtained. PCR products were directly sequenced (GenBank Accession No. EU308592), and a BLAST search in GenBank showed 98% nucleotide identity with published sequences of WVMV (GenBank Accession Nos. AF484549 and AY656816). In addition, RNA was extracted from the original isolate of WVMV that was reported in the Netherlands (1; supplied by R. van der Vlugt, Plant Research International) and the RNA was amplified using the WVMV-specific primer pair. The sequence obtained from PCR amplicons of the type isolate (GenBank Accession No. EU308593) showed a 98% nucleotide identity with the New Zealand WVMV isolate and with published sequences of WVMV (as shown above). From the symptomatology, particle morphology, and nucleotide sequences, it is concluded that WVMV is present in New Zealand. The distribution of the virus in New Zealand is not known, but the affected plants at the New Plymouth nursery may have been imported into New Zealand as many as 30 years ago. Although WVMV infection can reduce the quality of commercial plants, the disease is not economically significant in New Zealand. References: (1) L. Bos. Neth. J. Plant Pathol. 76:8, 1970. (2) G. R. G. Clover et al. Plant Pathol. 52:92, 2003. (3) S. A. Langeveld et al. J. Gen Virol. 72:1531, 1991. (4) A. M. Mackenzie et al. Arch Virol. 143:903, 1998.
紫藤脉花叶病毒（WVMV）是马铃薯Y病毒属的成员。该病毒已在澳大利亚、中国、美国以及一些欧洲国家的紫藤属植物中被报道（2）。2006年，在新西兰奥克兰以北怀努阿派的一个商业苗圃中，观察到几株具有斑驳和花叶症状的中华紫藤植株。这些植株是从新西兰新普利茅斯地区的一个苗圃繁殖而来的。用电子显微镜检查了有症状的紫藤植株的汁液，观察到了大约750纳米长的伸长且弯曲的类马铃薯Y病毒颗粒。使用Qiagen RNeasy植物迷你试剂盒（澳大利亚唐卡斯特）从有症状的植株中提取RNA。最初使用通用的马铃薯Y病毒引物PV1/SP6（4）和U335（3）对RNA进行检测，循环条件为94℃ 5分钟，随后40个循环，94℃ 45秒、50℃ 45秒、72℃ 90秒，最后72℃延伸7分钟。聚合酶链反应（PCR）产物（695碱基对）直接测序（GenBank登录号EU580146），在GenBank中进行的BLAST搜索显示与WVMV（GenBank登录号AF484549）有98%的核苷酸同一性。然后使用WVMV特异性引物WVMVF1和WVMVR1以及已发表的循环条件（2）对RNA进行检测。获得了701碱基对的PCR扩增产物。PCR产物直接测序（GenBank登录号EU308592），在GenBank中进行的BLAST搜索显示与已发表的WVMV序列（GenBank登录号AF484549和AY656816）有98%的核苷酸同一性。此外，从荷兰报道的WVMV原始分离株（1；由国际植物研究公司的R. van der Vlugt提供）中提取RNA，并使用WVMV特异性引物对进行扩增。从该典型分离株的PCR扩增产物获得的序列（GenBank登录号EU308593）显示与新西兰WVMV分离株以及已发表的WVMV序列有98%的核苷酸同一性（如上所示）。从症状学、颗粒形态和核苷酸序列来看，可以得出结论，WVMV存在于新西兰。该病毒在新西兰的分布情况未知，但新普利茅斯苗圃中受影响的植株可能在多达30年前就已被引入新西兰。尽管WVMV感染会降低商业植物的质量，但在新西兰该病害在经济上并不重要。参考文献：（1）L. Bos.《荷兰植物病理学杂志》76:8，1970。（2）G. R. G. Clover等人。《植物病理学》52:92，2003。（3）S. A. Langeveld等人。《普通病毒学杂志》72:1531，1991。（4）A. M. Mackenzie等人。《病毒学档案》143:903，1998。
