Chai Liang, Li Haojie, Zhao Xiaoguang, Cui Cheng, Zheng Benchuan, Zhang Ka, Jiang Jun, Zhang Jinfang, Jiang Liangcai
Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.
Environment-Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Chengdu 610066, China.
Plants (Basel). 2023 Jun 23;12(13):2429. doi: 10.3390/plants12132429.
Based on previous researches, we further investigated the multi-silique trait in rapeseed ( L.) line zws-ms. In this study, we used a relatively comprehensive list of flowering related genes in rapeseed and compared them between zws-ms and its near-isogenic line (NIL) zws-217. Genes were studied on genome, transcriptome and proteome levels and then we focused on genes with non-synonymous single nucleotide polymorphism (SNP) or frame-shift insertion-deletion (InDel), finding some genes on the list which changes their sequences. Then, combined with their annotation and the information of their orthologs, certain genes such as BnaA09g05900D, ortholog of (), which encodes an MADS-box protein, were assumed as probably responsible for the multi-silique trait. Also, we analyzed the Differentially Accumulated Proteins (DAPs) between zws-ms and zws-217, revealing some genes involved in homologous recombination and mismatch repair pathways. Since the development of flowers/siliques is crucial to crops and it influences the yield of rapeseed, this study paved a way to deeply understand the mechanism of the multi-pistil flower formation, which may facilitate researches on rapeseed production in future.
基于先前的研究，我们进一步研究了油菜（L.）品系zws-ms的多角果性状。在本研究中，我们使用了一份相对全面的油菜开花相关基因列表，并在zws-ms及其近等基因系（NIL）zws-217之间进行了比较。我们在基因组、转录组和蛋白质组水平上对基因进行了研究，然后重点关注具有非同义单核苷酸多态性（SNP）或移码插入缺失（InDel）的基因，在列表中发现了一些序列发生变化的基因。然后，结合它们的注释及其直系同源基因的信息，某些基因，如编码MADS-box蛋白的（）的直系同源基因BnaA09g05900D，被认为可能与多角果性状有关。此外，我们分析了zws-ms和zws-217之间的差异积累蛋白（DAP），揭示了一些参与同源重组和错配修复途径的基因。由于花/角果的发育对作物至关重要，并且它影响油菜的产量，本研究为深入了解多雌蕊花形成的机制铺平了道路，这可能有助于未来油菜生产的研究。
