Guizhou Key Laboratory of Mountain Environment, Guizhou Normal University, Guiyang, 550001, China.
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China.
Sci Rep. 2019 Feb 15;9(1):2160. doi: 10.1038/s41598-018-36886-z.
The interaction between soil property and soil microbial community in karst area still remains an open question. The characteristics of soil physicochemical properties and microbial community structure and their relationship under five vegetation succession stages (grassland, shrub land, secondary forest, plantation forest, and natural forest) at two soil depths (0-10 cm and 10-20 cm) were explored in a karst mountain ecosystem. We found that soil moisture content (SMC) and pH increased with soil depth across vegetation succession. The highest content of soil nutrients was found in the natural forest stage at both soil depths. The total PLFAs, the abundance of Gram-positive (GP) bacteria, actinomycetes (ACT), fungi, and arbuscular mycorrhizal fungi (AMF) were significantly (P < 0.05) related to variations with soil total carbon (TC) and total nitrogen (TN). Furthermore, the distribution of soil microbial community distinctly differed in vegetation succession both at two soil layers which was demonstrated by Principal-coordinates analysis. Redundancy analyses patterns indicated that soil TC and TN were positively related to cy19:0 and 10Me 16:0, but an opposite relationship with a15:0. Changes of soil microbial communities were significantly determined by vegetation succession, and soil microbial community structure can be a sensitive indicator to reflect the stabilization of karst mountain ecosystem, southwest of China.
喀斯特地区土壤性质与土壤微生物群落的相互作用仍然是一个悬而未决的问题。本研究在喀斯特山地生态系统中，探索了五个植被演替阶段（草地、灌木林、次生林、人工林和天然林）下两个土壤深度（0-10cm 和 10-20cm）的土壤理化性质和微生物群落结构特征及其相互关系。结果表明，土壤水分含量（SMC）和 pH 值随植被演替而增加。在两个土壤深度上，土壤养分含量最高的是天然林阶段。总 PLFAs、革兰氏阳性（GP）细菌、放线菌（ACT）、真菌和丛枝菌根真菌（AMF）的丰度与土壤总碳（TC）和总氮（TN）的变化显著相关（P<0.05）。此外，土壤微生物群落的分布在两个土层的植被演替中明显不同，这可以通过主坐标分析来证明。冗余分析模式表明，土壤 TC 和 TN 与 cy19:0 和 10Me 16:0 呈正相关，但与 a15:0 呈负相关。土壤微生物群落的变化主要由植被演替决定，土壤微生物群落结构可以作为反映中国西南喀斯特山地生态系统稳定的敏感指标。
