Tabatabaei Seyed Hosein, Sheikhha Mohammad Hasan, Karbasi Mahammad Hasan Akhavan, Zarmehi Sedighe, Hoseini Mehdi
Department of Oral and Maxillofacial Pathology, Member of Social Determinants of Oral Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Genetics, Research and Clinical Center for Infertility, Shahid Sadoughi Medical Sciences University, Yazd, Iran.
J Dent Res Dent Clin Dent Prospects. 2018 Fall;12(4):245-251. doi: 10.15171/joddd.2018.038. Epub 2018 Dec 19.
Our research was aimed to study protein codon 72 polymorphism, a single base pair change of either arginine (Arg; CGC) or proline (Pro; CCC) that creates 3 distinct genotypes in reticular oral lichen planus (OLP) in comparison to oral SCC which is the most common oral mucosal malignancy as positive control and inflammatory fibrous hyperplasia (IFH) lesion as negative control. Seventy paraffin-embedded tissue samples (30 OLP, 20 OSCC and 20 IFH) were studied. DNA was purified and amplified using allele-specific polymerase chain reaction to detect polymorphism. The final amplified products were identified using gel electrophoresis. Data were analyzed using chi-squared test and odds ratio. The mean ages in the OLP, OSCC and IFH groups were 43.28, 58.2 and 53.47 years, respectively, with significant differences. There were no significant differences in gender between the three groups (P=0.413); however, the differences in genotypes and alleles were significant between the three groups (P=0.021 and P=0.030, respectively). By considering IFH as a reference, the frequency of proline allele in OLP and OSCC was significantly higher than that of arginine allele (P=0.015 and P=0.028, respectively). In addition, by considering OSCC as a reference and at P=1, there were no significant differences in the frequencies of alleles between OSCC and OLP. The results might indicate the premalignant potential of OLP, and such polymorphism might be a genetic predisposing factor for conversion of OLP to OSCC. In addition, in the subjects evaluated the proline allele was considered a risk factor.
我们的研究旨在研究蛋白质密码子72多态性，即精氨酸（Arg；CGC）或脯氨酸（Pro；CCC）的单碱基对变化，该变化在网状口腔扁平苔藓（OLP）中产生3种不同的基因型，以最常见的口腔黏膜恶性肿瘤口腔鳞状细胞癌（OSCC）作为阳性对照，炎症性纤维增生（IFH）病变作为阴性对照。研究了70个石蜡包埋组织样本（30个OLP、20个OSCC和20个IFH）。使用等位基因特异性聚合酶链反应纯化并扩增DNA以检测多态性。使用凝胶电泳鉴定最终的扩增产物。使用卡方检验和比值比分析数据。OLP、OSCC和IFH组的平均年龄分别为43.28岁、58.2岁和53.47岁，存在显著差异。三组之间的性别无显著差异（P = 0.413）；然而，三组之间的基因型和等位基因差异显著（分别为P = 0.021和P = 0.030）。以IFH作为参照，OLP和OSCC中脯氨酸等位基因的频率显著高于精氨酸等位基因（分别为P = 0.015和P = 0.028）。此外，以OSCC作为参照且P = 1时，OSCC和OLP之间的等位基因频率无显著差异。结果可能表明OLP具有恶变潜能，这种多态性可能是OLP转化为OSCC的遗传易感因素。此外，在所评估的受试者中，脯氨酸等位基因被认为是一个危险因素。
