Taghipour Ali, Azimi Taher, Javanmard Ehsan, Pormohammad Ali, Olfatifar Meysam, Rostami Ali, Tabarsi Payam, Sohrabi Mohammad Reza, Mirjalali Hamed, Haghighi Ali
Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical sciences, Tehran, Iran.
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Gastroenterol Hepatol Bed Bench. 2018 Winter;11(Suppl 1):S134-S139.
To investigate the presence of intestinal parasites in tuberculosis patients who suffered from immunodeficiency disorders.
Tuberculosis is an important infectious disease that is endemic in some regions of Iran. However, there is a coverage in the endemicity areas of this infection with intestinal parasites.
Stool samples were collected from 50 immunocompromised tuberculosis patients. Direct smear using the normal saline (0.85% NaCl solution) and Lugol's iodine staining were performed to detect trophozoite of parasites. Moreover, stool samples were concentrated using routine formalin-ether to detect protozoan cysts and helminth's ova/larvae. Specific staining techniques including Trichrome, Modified Ziehl-Neelsen and chromotrope 2R were employed to detect amoeba, spp., coccidian parasites and microsporidia.
From 50 participants, 42 (84%) and 8 (16%) were male and female, respectively. The mean age + SD of patients was 47.88 + 10.88 years. Among the participated patients, HIV positive, cancer, organ transplant and receiving corticosteroids were seen in 13, 10, 15 and 12 subjects, respectively. The prevalence of Intestinal parasites was 34 %( 17/50). (18%; 9/50), and intestinal helminth () (2%; 1/50) were the most prevalent and less prevalent parasites, respectively. Statistical significance difference was not seen between presence of intestinal parasites and type of immunodeficiency.
Our findings showed the high prevalence of intestinal parasites with majority of . Indeed, this study suggested that due to complicated immune conditions of TB patients with immunodeficiency disorders, this group of patients are at higher risk of infection by intestinal parasites.
调查患有免疫缺陷疾病的结核病患者肠道寄生虫的感染情况。
结核病是一种重要的传染病，在伊朗的一些地区呈地方性流行。然而，在这种感染的地方性流行地区，肠道寄生虫的感染情况尚不清楚。
收集50例免疫功能低下的结核病患者的粪便样本。使用生理盐水（0.85%氯化钠溶液）进行直接涂片和卢戈氏碘染色以检测寄生虫滋养体。此外，使用常规甲醛-乙醚法对粪便样本进行浓缩，以检测原生动物囊肿和蠕虫虫卵/幼虫。采用包括三色染色、改良齐-尼氏染色和变色酸2R染色等特殊染色技术检测阿米巴原虫、隐孢子虫属、球虫寄生虫和微孢子虫。
50名参与者中，男性42名（84%），女性8名（16%）。患者的平均年龄±标准差为47.88±10.88岁。在参与研究的患者中，分别有13例、10例、15例和12例患者为HIV阳性、患有癌症、接受过器官移植和正在接受皮质类固醇治疗。肠道寄生虫的感染率为34%（17/50）。其中，隐孢子虫（18%；9/50）是最常见的寄生虫，而肠道蠕虫（2%；1/50）是最不常见的寄生虫。肠道寄生虫的感染与免疫缺陷类型之间无统计学显著差异。
我们的研究结果显示肠道寄生虫感染率很高，其中隐孢子虫最为常见。事实上，本研究表明，由于患有免疫缺陷疾病的结核病患者免疫状况复杂，这组患者感染肠道寄生虫的风险更高。
