埃及曼苏拉医院获得性尿路感染中高水平氨基糖苷类耐药肠球菌
High level aminoglycoside resistant enterococci in hospital-acquired urinary tract infections in Mansoura, Egypt.
作者信息
El-Mahdy Rasha, Mostafa Ahmed, El-Kannishy Ghada
机构信息
MD, Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.
MSc, Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.
出版信息
Germs. 2018 Dec 3;8(4):186-190. doi: 10.18683/germs.2018.1145. eCollection 2018 Dec.
INTRODUCTION
Resistance to different antimicrobial agents is increasing in enterococci and effective treatment represents a major health concern. The aim of this study was to determine the antimicrobial resistance patterns and the frequency of high level aminoglycoside resistance (HLAR) among enterococci.
METHODS
A total of 80 enterococcal isolates, (73 7 ) were collected from patients with hospital acquired urinary tract infections (UTI) at Mansoura University hospitals in Egypt. Antimicrobial susceptibility testing was performed via the disc diffusion method. PCR was used for identification of species and detection of aminoglycoside-modifying enzymes genes (AME).
RESULTS
All enterococcal isolates were sensitive to vancomycin and linezolid. Fifty-three isolates exhibited HLAR. Our results show that HLAR was mediated by the presence of multiple AMEs genes. The '' gene was associated with ' and gene in 69% of HLAR isolates.
CONCLUSION
This study showed that enterococci isolated from hospital acquired UTI were resistant to multiple antibiotics. Furthermore, the frequency of high level gentamicin resistance (HLGR) was higher than high level of streptomycin resistance (HLSR). The most common AME genes were ' and followed by ''
引言
肠球菌对不同抗菌药物的耐药性正在增加，有效的治疗成为一个主要的健康问题。本研究的目的是确定肠球菌的抗菌耐药模式以及高水平氨基糖苷类耐药（HLAR）的频率。
方法
从埃及曼苏拉大学医院的医院获得性尿路感染（UTI）患者中总共收集了80株肠球菌分离株（73株[7]）。通过纸片扩散法进行抗菌药敏试验。采用聚合酶链反应（PCR）鉴定菌种并检测氨基糖苷类修饰酶基因（AME）。
结果
所有肠球菌分离株对万古霉素和利奈唑胺敏感。53株分离株表现出HLAR。我们的结果表明，HLAR是由多种AME基因的存在介导的。在69%的HLAR分离株中，“基因与‘和’基因相关。
结论
本研究表明，从医院获得性UTI分离出的肠球菌对多种抗生素耐药。此外，高水平庆大霉素耐药（HLGR）的频率高于高水平链霉素耐药（HLSR）。最常见的AME基因是‘和’，其次是‘’
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