Moussa Ayan Aden, Md Nordin Amirah Fatihah, Hamat Rukman Awang, Jasni Azmiza Syawani
Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, UPM Serdang 43400, Malaysia.
Infect Drug Resist. 2019 Oct 21;12:3269-3274. doi: 10.2147/IDR.S219544. eCollection 2019.
and are among the predominant species causing hospital-acquired infections. Currently, enterococcal infections are treated using combination therapy of an aminoglycoside with cell-wall active agents, which led to high level aminoglycoside resistance (HLAR) and vancomycin resistance (VRE) among enterococci. The aim of this study was to determine the prevalence of HLAR and the distribution of the resistance genes among clinical and isolates in Malaysia.
Seventy-five enterococci isolates recovered from different clinical sources were re-identified by subculturing on selective medium, Gram staining, biochemical profiling (API 20 Strep), and 16s rRNA sequencing. Antimicrobial susceptibility testing (AST) was performed using Kirby-Bauer disc diffusion, E-test, and broth microdilution methods. PCR amplification was used to detect the presence of aminoglycoside modifying enzyme (AME) genes []. Descriptive data analysis was used to analyze the antibiotic susceptibility profiles and the distribution of HLAR genes.
The majority of the isolates recovered from the clinical samples are (66.7%), with the highest recovery from the pus. The prevalence of HLGR (51%) is higher when compared to HLSR (45-49%). Analysis of the resistance genes showed that bifunctional genes and contributed to the HLAR and . The other AME genes [] were not detected in this study.
This study provides the first prevalence data on HLAR and the distribution of the AME genes among and isolates from Malaysia. These highlight the need for continued antibiotic surveillance to minimize its emergence and further dissemination.
[具体菌种1]和[具体菌种2]是引起医院获得性感染的主要菌种。目前，肠球菌感染采用氨基糖苷类药物与细胞壁活性剂联合治疗，这导致肠球菌中出现高水平氨基糖苷类耐药（HLAR）和万古霉素耐药（VRE）。本研究的目的是确定马来西亚临床[具体菌种1]和[具体菌种2]分离株中HLAR的流行情况以及耐药基因的分布。
从不同临床来源分离出的75株肠球菌，通过在选择性培养基上进行传代培养、革兰氏染色、生化分析（API 20 Strep）和16s rRNA测序进行重新鉴定。采用 Kirby-Bauer 纸片扩散法、E-test法和肉汤微量稀释法进行药敏试验（AST）。采用PCR扩增检测氨基糖苷类修饰酶（AME）基因[]的存在。采用描述性数据分析抗生素敏感性谱和HLAR基因的分布。
从临床样本中分离出的大多数菌株是[具体菌种1]（66.7%），其中脓液样本中的分离株回收率最高。与高水平链霉素耐药（HLSR，45 - 49%）相比，高水平庆大霉素耐药（HLGR，51%）的患病率更高。耐药基因分析表明，双功能基因[]导致了HLAR[具体菌种1]和[具体菌种2]。本研究未检测到其他AME基因[]。
本研究提供了马来西亚[具体菌种1]和[具体菌种2]分离株中HLAR的首次流行数据以及AME基因的分布。这些结果凸显了持续进行抗生素监测以尽量减少其出现和进一步传播的必要性。
