School of Pharmacy , Sungkyunkwan University , Suwon 16419 , Republic of Korea.
Korea Research Institute of Chemical Technology , Deajeon 34114 , Republic of Korea.
J Nat Prod. 2019 Apr 26;82(4):765-773. doi: 10.1021/acs.jnatprod.8b00665. Epub 2019 Feb 18.
Withania somnifera, commonly known as "Indian ginseng" or "ashwagandha", is popular as a functional food because of its diverse purported therapeutic efficacies including invigorating, improvement of cognitive ability, and stress release activities. Chemical investigation of the MeOH extract of W. somnifera roots combined with LC/MS-based analysis resulted in the identification of six new withanolides, withasilolides A-F (1-6), as well as seven known compounds (7-13). The structures of the new compounds were established by application of spectroscopic methods, including 1D and 2D NMR, HRMS, and ECD measurements. The cytotoxicity of the isolated compounds was evaluated against four human cancer cell lines (A549, SK-OV-3, SK-MEL-2, and HCT-15). Compounds 1, 2, 4, 6, and withanone (11) each showed cytotoxicity for one or more of the four cancer cell lines used.
睡茄，俗称“印度人参”或“冬虫草”，因其具有多种声称的治疗功效而被广泛用作功能性食品，包括滋补、改善认知能力和缓解压力的功效。对睡茄根的 MeOH 提取物进行化学研究，并结合 LC/MS 分析，鉴定出 6 种新的 withanolide，分别为 withasilolides A-F（1-6），以及 7 种已知化合物（7-13）。通过应用包括 1D 和 2D NMR、HRMS 和 ECD 测量在内的光谱方法确定了新化合物的结构。对分离得到的化合物进行了对 4 个人类癌细胞系（A549、SK-OV-3、SK-MEL-2 和 HCT-15）的细胞毒性评估。化合物 1、2、4、6 和 withanone（11）对使用的 4 种癌细胞系中的一种或多种均显示出细胞毒性。
