a Organic Synthesis Laboratory , Research and Development Institute - IPD Vale do Paraíba University , São José dos Campos , Brazil.
b Chemistry Department Photobiology and Photomedicine Group , University of São Paulo , Ribeirão Preto , São Paulo , Brazil.
J Biomater Sci Polym Ed. 2019 May;30(7):508-525. doi: 10.1080/09205063.2019.1580664. Epub 2019 Mar 20.
In this study, zinc phthalocyanine (ZnPc) was loaded onto gelatin nanoparticles functionalized with polyelectrolytes (polystyrene sulfonate/polyallylamine hydrochloride) by layer-by-layer (LbL) assembly. The process yield and the encapsulation efficiency were 76.0% ± 2.5 and 86.0% ± 1.8, respectively. The functionalized photosensitive gelatin nanoparticles (FPGN) had a mean diameter of 396.5 ± 45.8 nm, narrow distribution size with a polydispersity index of 0.106. The obvious switching of zeta potential indicates successful alternating deposition of the polyanion PSS and polycation PAH directly on the gelatin nanoparticles. The in vitro drug release investigation found that the LbL deposited polyelectrolyte bilayer is very efficient to reduce the release rate and assuage the initial burst for drugs loaded in gelatin nanoparticles. The photobiological activity of FPGN was evaluated on mouse macrophage carcinoma line J774 A-1. The cells viability decreased with the increase of the light dose in the range of 1-10.0 J.cm. ZnPc-loaded in functionalized gelatin nanoparticles are the release systems that promise photodynamic therapy use.
在这项研究中，锌酞菁（ZnPc）通过层层（LbL）组装负载到带聚电解质（聚苯乙烯磺酸钠/盐酸聚烯丙胺）的明胶纳米颗粒上。该过程的产率和包封效率分别为 76.0%±2.5%和 86.0%±1.8%。功能化光敏明胶纳米颗粒（FPGN）的平均直径为 396.5±45.8nm，具有较窄的分布尺寸和 0.106 的多分散指数。明显的zeta 电位变化表明，带负电荷的 PSS 和带正电荷的 PAH 聚电解质可以直接在明胶纳米颗粒上交替沉积。体外药物释放研究发现，LbL 沉积的聚电解质双层对负载在明胶纳米颗粒中的药物的释放速率有非常有效的降低作用，并减轻了药物的初始突释。FPGN 的光生物活性在小鼠巨噬细胞肉瘤系 J774 A-1 上进行了评估。细胞活力随着光剂量在 1-10.0 J.cm 的范围内增加而降低。负载在功能化明胶纳米颗粒中的 ZnPc 是一种有前途的光动力治疗释放系统。
