Laue Hannah E, Brennan Kasey J M, Gillet Virginie, Abdelouahab Nadia, Coull Brent A, Weisskopf Marc G, Burris Heather H, Zhang Wei, Takser Larissa, Baccarelli Andrea A
Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
Department of Environmental Health Sciences, Columbia Mailman School of Public Health, New York, NY, USA.
Environ Epidemiol. 2019 Feb;3(1). doi: 10.1097/EE9.0000000000000039.
The gut microbiome is influenced by early-life exposures, but-despite potentially enormous implications for child health-is understudied in environmental epidemiology. This pilot study is one of the first to explore exposures and long-term gut microbiome profiles. We examined the association between exposure to polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) during pregnancy and the mid-childhood gut microbiome.
We measured levels of PBDE-47, -99, -100, and -153 and PCB-138, -153, and -180 in maternal plasma during early pregnancy (n=18) and at delivery (n=25) in women of European descent who breastfed the child participant of the Gestation and Environment cohort in Sherbrooke, Québec (recruited 2007-2009). Bacteria in the mid-childhood (6-8 years) fecal microbiome were detected with 16S rRNA sequencing. To test for differences at the taxon level, we used the Microbiome Comprehensive Association Mapping algorithm.
Early pregnancy PCB-153, -180, and the sum of PCBs (ΣPCB) concentrations were associated with a higher relative abundance of Propionibacteriales and Propionibacteriaceae in mid-childhood. Higher PCB-180 and ΣPCB were associated with higher relative abundance of Bacillales Family XI. Higher PBDE-99 exposure was associated with a decrease in uncultured bacteria within the Ruminococcaceae NK4A214 group and PBDE-47 was associated with differences in . These taxon-level changes did not result in differences in within- or between-subject diversity. Exposures at delivery were not associated with differences in taxa.
Prenatal exposure to PCBs and PBDEs is associated with mid-childhood gut microbiome profiles. Larger studies are needed to confirm these results and explore health implications.
肠道微生物群会受到早期生活暴露的影响，但尽管这可能对儿童健康产生巨大影响，在环境流行病学中却未得到充分研究。这项试点研究是最早探索暴露与长期肠道微生物群特征的研究之一。我们研究了孕期接触多溴二苯醚（PBDEs）和多氯联苯（PCBs）与儿童中期肠道微生物群之间的关联。
我们测量了魁北克省舍布鲁克市妊娠与环境队列中参与研究的儿童的母亲（2007 - 2009年招募，欧洲血统）孕早期（n = 18）和分娩时（n = 25）母血中PBDE - 47、 - 99、 - 100和 - 153以及PCB - 138、 - 153和 - 180的水平。通过16S rRNA测序检测儿童中期（6 - 8岁）粪便微生物群中的细菌。为了检测分类群水平的差异，我们使用了微生物群综合关联图谱算法。
孕早期PCB - 153、 - 180以及多氯联苯总和（ΣPCB）浓度与儿童中期丙酸杆菌目和丙酸杆菌科的相对丰度较高有关。较高的PCB - 180和ΣPCB与芽孢杆菌科XI的相对丰度较高有关。较高的PBDE - 99暴露与瘤胃球菌科NK4A214组内未培养细菌的减少有关，PBDE - 47与[此处原文缺失部分内容]的差异有关。这些分类群水平的变化并未导致个体内或个体间多样性的差异。分娩时的暴露与分类群差异无关。
产前接触多氯联苯和多溴二苯醚与儿童中期肠道微生物群特征有关。需要更大规模的研究来证实这些结果并探索其对健康的影响。
