Grech-Angelini Sébastien, Richomme Céline, Peytavin de Garam Carine, Boucher Jean-Marc, Maestrini Oscar, Grenouillet Frédéric, Casabianca François, Boué Franck, Umhang Gérald
INRA, UR045, Laboratoire de Recherches sur le Développement de l'Elevage (Research Laboratory for the Development of Livestock), Corte, France.
Groupement Technique Vétérinaire de Corse, Ghisonaccia, France.
Parasitol Res. 2019 Apr;118(4):1313-1319. doi: 10.1007/s00436-019-06261-6. Epub 2019 Feb 18.
Recent surveys at slaughterhouses confirmed the presence of three different species of Echinococcus granulosus sensu lato in France: E. granulosus sensu stricto, E. ortleppi, and E. canadensis G6/7. The latter species was only identified on the French Mediterranean island of Corsica, with a high prevalence in pigs and wild boar. In order to investigate the life cycle of E. canadensis in this region, dog feces were collected in 31 municipalities, mainly from individual kennels. The analysis of fecal samples from 259 dogs by multiplex real-time PCR shows no infection by E. granulosus sensu stricto, but three dogs were infected by E. canadensis G6/7. Genetic analyses of mitochondrial genes (cox1, nad1, nad3, atp6) revealed in two dogs a haplotype previously identified in pigs. The third dog was infected by a new haplotype differing only from the two others from dogs by two mutations in the nad3 gene. This latter haplotype is genetically closer to those identified in pigs rather than those from wild boars. Analysis of questionnaires completed by the owners revealed that the sampled dog population was almost exclusively composed of hunting dogs that had been infrequently dewormed. Most of the owners (78%) leave carcasses of hunter-harvested wild boar in close proximity to their dogs. Nevertheless, genetic results seem to indicate that the three dogs were infected due to their consumption of a pig's infected viscera following home slaughtering. This study confirms the role of dogs as definitive hosts of E. canadensis G6/7 in Corsica. Further molecular studies, notably in human cases, are needed to assess the zoonotic impact of E. canadensis G6/7 in this region.
最近在屠宰场进行的调查证实，法国存在三种不同的细粒棘球绦虫广义种：狭义细粒棘球绦虫、奥氏棘球绦虫和加拿大棘球绦虫G6/7。后一种仅在法国地中海岛屿科西嘉岛被发现，在猪和野猪中的感染率很高。为了调查该地区加拿大棘球绦虫的生命周期，在31个市镇收集了狗粪便，主要来自个体犬舍。通过多重实时PCR对259只狗的粪便样本进行分析，结果显示没有狭义细粒棘球绦虫感染，但有三只狗感染了加拿大棘球绦虫G6/7。对线粒体基因（cox1、nad1、nad3、atp6）的遗传分析显示，两只狗的单倍型先前已在猪中鉴定出。第三只狗感染了一种新的单倍型，该单倍型与另外两只狗的单倍型仅在nad3基因上有两个突变。后一种单倍型在基因上与猪中鉴定出的单倍型更接近，而不是与野猪中的单倍型接近。对主人填写的问卷进行分析发现，抽样的狗群几乎完全由很少驱虫的猎犬组成。大多数主人（78%）将猎人捕获的野猪尸体留在离他们的狗很近的地方。然而，基因结果似乎表明，这三只狗是因为食用了家庭屠宰后感染的猪内脏而被感染的。这项研究证实了狗作为科西嘉岛加拿大棘球绦虫G6/7终末宿主的作用。需要进一步的分子研究，特别是在人类病例中，以评估加拿大棘球绦虫G6/7在该地区的人畜共患病影响。
