Wang Li-Ping, Peng Xiao-Yu, Lv Xiao-Qin, Liu Lin, Li Xue-Li, He Xiao, Lv Fang, Pan Yu, Wang Li, Liu Kai-Feng, Zhang Xiao-Mei
Department of Biobank, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu, China.
Northern Jiangsu People's Hospital, Yangzhou University, Yangzhou, Jiangsu, China.
J Cell Physiol. 2019 Sep;234(9):15537-15547. doi: 10.1002/jcp.28201. Epub 2019 Feb 18.
Polycystic ovary syndrome (PCOS) is one of the most prevalent reproductive disorders in women worldwide. Despite rigorous research, the exact molecular mechanism that governs PCOS pathogenesis remains unclear. To investigate the potential roles of circular RNAs (circRNAs), this study sequenced ribosomal RNA-depleted total RNA from exosomes of follicle fluids obtained from PCOS patients using non-PCOS samples as controls. Bioinformatic analysis identified 167 upregulated and 245 downregulated circRNAs from a total of 16,771 detected candidates. Functional analysis suggests that pathways related to bacterial infection, associated chronic inflammation, and oxidative stress could be targeted by the differential circRNAs in PCOS patients. The obtained sequencing results were further validated by quantitative reverse-transcription polymerase chain reaction and a circRNA-microRNA interaction network was constructed. The obtained results provide a valuable addition to the published studies on the mechanism of PCOS pathogenesis by revealing a wide variety of new circRNAs, miRNA, and gene targets that merit further investigation.
多囊卵巢综合征（PCOS）是全球女性中最常见的生殖系统疾病之一。尽管进行了大量研究，但PCOS发病机制的确切分子机制仍不清楚。为了研究环状RNA（circRNA）的潜在作用，本研究以非PCOS样本为对照，对PCOS患者卵泡液外泌体中去除核糖体RNA的总RNA进行了测序。生物信息学分析从总共16771个检测到的候选物中鉴定出167个上调的circRNA和245个下调的circRNA。功能分析表明，与细菌感染、相关慢性炎症和氧化应激相关的通路可能是PCOS患者差异circRNA的作用靶点。通过定量逆转录聚合酶链反应进一步验证了获得的测序结果，并构建了circRNA- microRNA相互作用网络。通过揭示各种值得进一步研究的新的circRNA、miRNA和基因靶点，所得结果为已发表的关于PCOS发病机制的研究提供了有价值的补充。
