Department of Chemistry, Loyola Science Center , University of Scranton , 925 Ridge Row , Scranton , Pennsylvania 18510 , United States.
Avignon University, INRA, UMR408 SQPOV , 84000 Avignon , France.
J Agric Food Chem. 2019 Aug 21;67(33):9139-9147. doi: 10.1021/acs.jafc.8b06674. Epub 2019 Feb 28.
Cardiovascular disease (CVD) is the leading cause of death in industrialized nations. The initiating event in atherosclerosis, or hardening of the arteries, is oxidation of low density lipoprotein (LDL). Binding with serum albumin and LDL of 41 polyphenols (major antioxidants in plant foods) constituting four classes of flavonoids, three types of phenolic acids, and seven polyphenol conjugate metabolites was investigated indirectly by fluorescence quenching and directly by affinity separation/high-performance liquid chromatography (four of the polyphenols). Stern-Volmer plots yielded values for the two proteins. Polyphenol binding was significantly stronger for albumin than with LDL. values were highly correlated with the lipophilicity of the polyphenols. The number of polyphenol molecules determined by quenching was ∼1 for both proteins. Direct analysis under saturation conditions yielded from 2 to 13 molecules of polyphenols/LDL particle. Multiple substituent effects on binding were analyzed. Evidence was put forward that binding of polyphenols to these proteins is protective for CVD by multiple mechanisms.
心血管疾病（CVD）是工业化国家的主要死因。动脉粥样硬化或动脉硬化的起始事件是低密度脂蛋白（LDL）的氧化。通过荧光猝灭间接研究了 41 种多酚（植物性食物中的主要抗氧化剂）与血清白蛋白和 LDL 的结合，这 41 种多酚分为四类类黄酮、三种类型的酚酸和七种多酚共轭代谢物，并通过亲和分离/高效液相色谱法直接进行了研究（其中四种多酚）。Stern-Volmer 图得出了两种蛋白质的 值。多酚与白蛋白的结合明显强于 LDL。 值与多酚的亲脂性高度相关。通过猝灭确定的多酚分子数约为每个蛋白质 1 个。在饱和条件下直接分析得到每个 LDL 颗粒结合 2 到 13 个多酚分子。分析了对结合的多重取代基效应。提出了多酚与这些蛋白质的结合通过多种机制对 CVD 具有保护作用的证据。
