The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, 890-0056, Japan.
Faculty of Fisheries, Kagoshima University, Kagoshima, 890-0056, Japan.
Sci Rep. 2019 Feb 20;9(1):2383. doi: 10.1038/s41598-019-38908-w.
This study investigated whether irradiation of a specific light wavelength could affect the sex differentiation of fish. We first found that the photoreceptor genes responsible for receiving red, green, and ultraviolet light were expressed in the eyes of medaka during the sex differentiation period. Second, we revealed that testes developed in 15.9% of genotypic females reared under green light irradiation. These female-to-male sex-reversed fish (i.e. neo-males) showed male-specific secondary sexual characteristics and produced motile sperm. Finally, progeny tests using the sperm of neo-males (XX) and eggs of normal females (XX) revealed that all F1 offspring were female, indicating for the first time in animals that irradiation with light of a specific wavelength can trigger sex reversal.
这项研究探讨了特定波长的光照射是否会影响鱼类的性别分化。我们首先发现，在性分化期间，负责接收红光、绿光和紫外线的光受体基因在斑马鱼的眼睛中表达。其次，我们揭示了在绿光照射下饲养的基因型雌性中有 15.9%发育出了睾丸。这些雌性到雄性的性别反转鱼（即 neo-males）表现出雄性特有的第二性征，并产生有活力的精子。最后，使用 neo-males（XX）的精子和正常雌性（XX）的卵子进行的后代测试表明，所有 F1 后代均为雌性，这是首次在动物中证明特定波长的光照射可以引发性别反转。
