Department of Chemistry, Qom Branch, Islamic Azad University, Qom, Islamic Republic of Iran.
Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Islamic Republic of Iran.
Sci Rep. 2021 Sep 21;11(1):18734. doi: 10.1038/s41598-021-98133-2.
In recent year, metal-organic frameworks (MOFs) have been displayed to be a category of promising drug delivery systems because of their crystalline structure, the potential of further functionality, and high porosity. In this research, graphene oxide was synthesized from pure graphite via hummer method and then MgFeO nanoparticles was incorporated into the synthesized ZIF-8 metal-organic frameworks which followed with loading on the surfaces of graphene oxide. In continue, tetracycline as an antibiotic drug was loaded on the surfaces and the cavities of the prepared nanocomposite. The outcomes of this research revealed that 90% of the tetracycline was loaded on the synthesized ZIF-8/GO/MgFeO nanostructure. Next, drug release was done at pH: 5 and pH: 7.4 within 3 days, resulting about 88% and 92% release of the tetracycline, respectively. With using different spectroscopic methods like X-ray crystallography (XRD), scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDX/Mapping), Fourier transform infrared (FTIR), thermalgravimetric analysis (TGA), and Brunauer-Emmett-Teller (BET), the structure of synthesized materials was confirmed. Furthermore, the antibiotic activity of tetracycline trapped into the ZIF-8/GO/MgFeO was evaluated by agar-well diffusion method on both gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria, which showed good antibacterial results.
近年来，金属有机骨架（MOFs）因其晶体结构、进一步功能化的潜力和高孔隙率而被证明是一类有前途的药物传递系统。在这项研究中，通过 Hummer 法从纯石墨合成了氧化石墨烯，然后将 MgFeO 纳米颗粒掺入合成的 ZIF-8 金属有机骨架中，随后负载在氧化石墨烯的表面上。接下来，将四环素作为抗生素药物负载在制备的纳米复合材料的表面和空腔上。研究结果表明，90%的四环素被负载在合成的 ZIF-8/GO/MgFeO 纳米结构上。接下来，在 pH 值为 5 和 pH 值为 7.4 的条件下进行了 3 天的药物释放，分别释放了约 88%和 92%的四环素。使用不同的光谱方法，如 X 射线晶体学（XRD）、扫描电子显微镜（SEM）、能谱（EDX/Mapping）、傅里叶变换红外（FTIR）、热重分析（TGA）和 Brunauer-Emmett-Teller（BET），确认了合成材料的结构。此外，通过琼脂孔扩散法对负载在 ZIF-8/GO/MgFeO 中的四环素的抗生素活性进行了评估，结果表明对革兰氏阳性（金黄色葡萄球菌）和革兰氏阴性（大肠杆菌）细菌均具有良好的抗菌效果。
