Parra Edwin Roger, Francisco-Cruz Alejandro, Wistuba Ignacio Ivan
Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, 2130 West Holcombe Blvd, Houston, TX 77030, USA.
Cancers (Basel). 2019 Feb 20;11(2):247. doi: 10.3390/cancers11020247.
Multiplexed platforms for multiple epitope detection have emerged in the last years as very powerful tools to study tumor tissues. These revolutionary technologies provide important visual techniques for tumor examination in formalin-fixed paraffin-embedded specimens to improve the understanding of the tumor microenvironment, promote new treatment discoveries, aid in cancer prevention, as well as allowing translational studies to be carried out. The aim of this review is to highlight the more recent methodologies that use multiplexed staining to study simultaneous protein identification in formalin-fixed paraffin-embedded tumor tissues for immune profiling, clinical research, and potential translational analysis. New multiplexed methodologies, which permit the identification of several proteins at the same time in one single tissue section, have been developed in recent years with the ability to study different cell populations, cells by cells, and their spatial distribution in different tumor specimens including whole sections, core needle biopsies, and tissue microarrays. Multiplexed technologies associated with image analysis software can be performed with a high-quality throughput assay to study cancer specimens and are important tools for new discoveries. The different multiplexed technologies described in this review have shown their utility in the study of cancer tissues and their advantages for translational research studies and application in cancer prevention and treatments.
近年来，用于多表位检测的多重平台已成为研究肿瘤组织的强大工具。这些革命性技术为福尔马林固定石蜡包埋标本中的肿瘤检查提供了重要的可视化技术，以增进对肿瘤微环境的理解、促进新治疗方法的发现、助力癌症预防，并开展转化研究。本综述的目的是突出使用多重染色来研究福尔马林固定石蜡包埋肿瘤组织中同时进行蛋白质鉴定以用于免疫分析、临床研究和潜在转化分析的最新方法。近年来已开发出新型多重方法，能够在单个组织切片中同时鉴定多种蛋白质，具备研究不同肿瘤标本（包括全切片、芯针活检组织和组织微阵列）中不同细胞群体、逐个细胞及其空间分布的能力。与图像分析软件相关的多重技术可通过高质量通量检测来研究癌症标本，是实现新发现的重要工具。本综述中描述的不同多重技术已在癌症组织研究中展现出其实用性及其在转化研究以及癌症预防和治疗应用中的优势。
