Lin Shouer, Qiu Wenqian, Hua Yongyou, Yang Yan
Physical and Chemical Analysis Department, Fujian Provincial Center For Disease Control and Prevention, Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou, Fujian 350001, China.
Food Chem X. 2024 Nov 22;24:102032. doi: 10.1016/j.fochx.2024.102032. eCollection 2024 Dec 30.
Caine-based anesthetics are frequently used in the production and transportation of aquatic products, but residues in fish threaten human health. A rapid, sensitive, and effective method was developed for detecting caine-based anesthetics and their metabolite residues in fish using ultra-high performance liquid chromatography-tandem mass spectrometry coupled with a modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) method. The sample was extracted with 0.05 % () formic acid-80 % acetonitrile, 4 g NaCl was added for liquid partitioning, and 50 mg C was used for purification. Matrix-matched calibrations showed good correlation coefficients with R ≥ 0.9942. The limits of detection were 0.5-4.4 ng/g. The recoveries ranged from 71.4 %-115.8 %, and the relative standard deviation for intra-day and inter-day precision was less than 8.5 % and 9.2 %, respectively. This method effectively analyzed the residues of caine-based anesthetics and their metabolites in fish and could be applied to aquatic product anesthetic regulation.
基于卡因的麻醉剂常用于水产品的生产和运输，但鱼类中的残留会威胁人类健康。采用超高效液相色谱-串联质谱联用改进的QuEChERS（快速、简便、廉价、有效、耐用且安全）方法，开发了一种快速、灵敏且有效的检测鱼类中基于卡因的麻醉剂及其代谢物残留的方法。样品用0.05%（）甲酸-80%乙腈提取，加入4 g氯化钠进行液液分配，并用50 mg C进行净化。基质匹配校准显示具有良好的相关系数，R≥0.9942。检测限为0.5 - 4.4 ng/g。回收率在71.4% - 115.8%之间，日内和日间精密度的相对标准偏差分别小于8.5%和9.2%。该方法有效地分析了鱼类中基于卡因的麻醉剂及其代谢物的残留，可应用于水产品麻醉剂监管。
