Zoological Institute, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 4, 76131, Karlsruhe, Germany.
Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), Wolfgang-Gaede-Str. 1, 76131, Karlsruhe, Germany.
Adv Mater. 2019 Jun;31(26):e1808110. doi: 10.1002/adma.201808110. Epub 2019 Feb 21.
Mimicking the properties of the extracellular matrix is crucial for developing in vitro models of the physiological microenvironment of living cells. Among other techniques, 3D direct laser writing (DLW) has emerged as a promising technology for realizing tailored 3D scaffolds for cell biology studies. Here, results based on DLW addressing basic biological issues, e.g., cell-force measurements and selective 3D cell spreading on functionalized structures are reviewed. Continuous future progress in DLW materials engineering and innovative approaches for scaffold fabrication will enable further applications of DLW in applied biomedical research and tissue engineering.
模拟细胞外基质的特性对于开发活细胞生理微环境的体外模型至关重要。除其他技术外，3D 直接激光写入 (DLW) 已成为实现用于细胞生物学研究的定制 3D 支架的有前途的技术。在这里，基于解决基本生物学问题的 DLW 结果，例如细胞力测量和在功能化结构上选择性的 3D 细胞扩展进行了回顾。DLW 材料工程的持续未来进展和支架制造的创新方法将使 DLW 在应用于生物医学研究和组织工程中的应用得到进一步发展。
