Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina. Rodovia Celso Garcia Cid - Campus Universitário, CEP 86057-970 - Londrina, PO Box 10011, Paraná, Brazil.
Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina. Rodovia Celso Garcia Cid - Campus Universitário, CEP 86057-970 - Londrina, PO Box 10011, Paraná, Brazil; Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina. Rodovia Celso Garcia Cid - Campus Universitário, CEP 86057-970 - Londrina, PO Box 10011, Paraná, Brazil.
Virus Res. 2019 Apr 15;264:8-15. doi: 10.1016/j.virusres.2019.02.009. Epub 2019 Feb 20.
Canine distemper is a highly contagious systemic viral disease, with worldwide distribution that affects a wide variety of terrestrial carnivores. This study characterized full-length fusion (F) genes from 15 Brazilian wild-type canine distemper virus (CDV) strains collected between 2003-2004 (n = 6) and 2013-2016 (n = 9). Using deduced amino acid (aa) sequence analysis, 14 strains were classified into Europe 1/South America 1 (EU1/SA1) lineage, with a temporal clustering into past (2003-2004) and contemporary (2013-2016) strains. One strain clustered to Rockborn-like lineage, showing high similarity (98.5%) with the Rockborn vaccine strain. In analyzed strains, the fusion protein signal-peptide (Fsp) coding region was highly variable at the aa level (67.4%-96.2%). The Brazilian strains were more Fsp-divergent from the North America 1 (NA1) strains (24.5%-36.3%) than from the Rockborn (11.2%-14.9%) vaccine strain. Seventeen cysteine residues in the full-length F gene and four non-conserved glycosylation sites in the Fsp region were detected. The results reveal that past and contemporary CDV strains are currently co-circulating. This first analysis of full-length F genes from Brazilian wild-type CDV strains contributes to knowledge of molecular epidemiology of CDV viral infection and evolution.
犬瘟热是一种具有全球分布的高度传染性全身病毒性疾病，影响多种陆地食肉动物。本研究对 2003-2004 年（n=6）和 2013-2016 年（n=9）间采集的 15 株巴西野生型犬瘟热病毒（CDV）的全长融合（F）基因进行了特征描述。通过推导的氨基酸（aa）序列分析，14 株被归类为欧洲 1/南美 1（EU1/SA1）谱系，存在过去（2003-2004 年）和当代（2013-2016 年）毒株的时间聚类。一株与 Rockborn 样谱系聚类，与 Rockborn 疫苗株具有高度相似性（98.5%）。在所分析的毒株中，融合蛋白信号肽（Fsp）编码区在 aa 水平上高度可变（67.4%-96.2%）。巴西株与北美 1（NA1）株（24.5%-36.3%）相比，与 Rockborn 疫苗株（11.2%-14.9%）的 Fsp 差异更大。全长 F 基因中有 17 个半胱氨酸残基，Fsp 区有 4 个非保守糖基化位点。结果表明，过去和当代 CDV 株目前共同循环。这是首次对巴西野生型 CDV 株全长 F 基因进行分析，有助于了解 CDV 病毒感染和进化的分子流行病学。
