Barbosa Vitória Sahena Martins Souza, Deus Danielle Rodrigues de, Bandeira Renato da Silva, Teixeira Dielle Monteiro, Maués Marcelino Antônio Costa, Figueiredo Márcia Janete de Fátima Mesquita de, Casseb Alexandre do Rosário, Silva Luciana Damascena da, Soares Luana Silva, Gabbay Yvone Benchimol, Siqueira Jones Anderson Monteiro, Resque Hugo Reis
Instituto Evandro Chagas (IEC), Ministério da Saúde, Ananindeua, Brazil.
Universidade Federal Do Oeste Do Pará (UFOPA), Ministério da Educação, Belém, Brazil.
Braz J Microbiol. 2025 Aug 13. doi: 10.1007/s42770-025-01753-3.
Canine distemper (CD) is a severe infectious disease of dogs caused by the Canine Distemper Virus (CDV), a member of the genus Morbillivirus. This study aimed to investigate the circulation of CDV in dogs housed in two public kennels located in Belém in the Amazon region of Brazil. Fecal and rectal swab samples were collected between March 2019 and April, 2020. A one-step RT-PCR was initially standardized for CDV detection in fecal samples. Positive strains were partially sequenced using Sanger sequencing. A prevalence rate of 23.6% was observed, with 18.4% in healthy dogs and 46.7% in dogs with diarrhea. Phylogenetic analysis revealed that the samples were closely related to European strains and demonstrated the circulation of Brazilian strains belonging to the Europe 1/South America 1 lineage. This investigation successfully established a CDV diagnostic technique using RT-PCR in blood, feces, and urine samples. This study also verified the circulation of the virus in dogs from public kennels in an important city in the Brazilian Amazon.
犬瘟热（CD）是由犬瘟热病毒（CDV）引起的犬类严重传染病，CDV属于麻疹病毒属。本研究旨在调查位于巴西亚马逊地区贝伦的两个公共犬舍中犬只的CDV流行情况。在2019年3月至2020年4月期间采集了粪便和直肠拭子样本。最初对一步法RT-PCR进行了标准化，用于粪便样本中CDV的检测。使用桑格测序法对阳性菌株进行了部分测序。观察到的患病率为23.6%，健康犬为18.4%，腹泻犬为46.7%。系统发育分析表明，这些样本与欧洲菌株密切相关，并证明了属于欧洲1/南美洲1谱系的巴西菌株的流行。本研究成功建立了一种利用RT-PCR检测血液、粪便和尿液样本中CDV的诊断技术。本研究还证实了该病毒在巴西亚马逊一个重要城市的公共犬舍犬只中的流行情况。
