Jones T C, Bienz K A, Erb P
Infect Immun. 1986 Jan;51(1):147-56. doi: 10.1128/iai.51.1.147-156.1986.
Long-term culturing of Toxoplasma gondii cysts was accomplished in vitro in association with murine astrocytes and intermittent additions of gamma interferon to the media. Phase-contrast microscopy was used to follow the stages of cyst development, and electron microscopy confirmed the presence of morphologic characteristics of T. gondii cysts. T. gondii cysts formed in vitro had a single trilaminar membrane during both intracellular and extracellular existence and contained amorphous electron-dense material either throughout the cyst or in a uniform layer under the trilaminar membrane. The bradyzoites were similar to previous descriptions of tachyzoites in vitro except that they were smaller and contained numerous electron-lucent vacuoles. Gamma interferon is not necessary for cyst formation, but it controls the division of tachyzoites and may allow cysts to remain for prolonged periods without rupturing. In vitro-cultivated T. gondii cysts will be useful for producing bradyzoite and cyst antigens and for measuring the effects of antimicrobial agents and immune modulators on the viability of intracystic T. gondii.
通过与小鼠星形胶质细胞联合培养，并向培养基中间歇添加γ干扰素，实现了体外长期培养刚地弓形虫包囊。利用相差显微镜观察包囊发育阶段，电子显微镜证实了刚地弓形虫包囊形态特征的存在。体外形成的刚地弓形虫包囊在细胞内和细胞外存在时均具有单层三层膜，并且在整个包囊内或三层膜下的均匀层中含有无定形电子致密物质。缓殖子与之前体外速殖子的描述相似，只是它们较小且含有许多电子透亮空泡。γ干扰素对于包囊形成并非必需，但它可控制速殖子的分裂，并可能使包囊长时间留存而不破裂。体外培养的刚地弓形虫包囊将有助于生产缓殖子和包囊抗原，并用于测定抗菌剂和免疫调节剂对包囊内刚地弓形虫活力的影响。
