Gori Pooja Veljibhai, Raval Manan, Patel Samir, Patel Amit, Solanki Nilay, Patel Priyal, Nandpal Manish, Mandal Palash, Aghara Hiral
Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology (CHARUSAT), CHARUSAT Campus, At and Post: Dist. Anand,, Changa, Gujarat, 388421, India.
Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology (CHARUSAT), CHARUSAT Campus, At and Post: Dist. Anand, Changa, Gujarat, 388421, India.
Mol Biol Rep. 2025 Jun 4;52(1):546. doi: 10.1007/s11033-025-10654-5.
Diabetes Mellitus (DM) impairs male reproductive functions by modulating apoptosis, oxidation, and the inflammatory pathway. The studies were planned to screen the ability of the hydro-alcoholic extract (AN) prepared using the roots of Argyreia nervosa (A. nervosa) (Burm.Fil.) Bojer. (Family: Convolvulaceae) in alleviating the DM-induced detrimental functioning of male reproductive functions in experimental rats.
Diabetic conditions were induced by the combined administration of a high-fat diet and a single injection of streptozotocin (30 mg/kg). Diabetic rats treated with a higher dose of A. nervosa extract (ANH) showed decreased fasting blood glucose (0.52-fold), abnormal sperm morphology (0.50-fold), and sperm DNA fragmentation index (0.74-fold), along with improved semen quality. ANH increased testicular testosterone (1.40-fold), FSH (1.91-fold), LH (1.69-fold), and mRNA expression of StAR (1.75-fold), 17β-HSD (1.77-fold), and 3β-HSD (1.06-fold). It reduced MDA (0.72-fold) and raised SOD (1.93-fold) and GSH (2.13-fold). ANH also lowered mRNA/protein levels of RAGE (0.75-fold, 8.81-fold), TNF-α (0.79-fold, 2.11-fold), NLRP3 (0.68-fold, 8.15-fold), and Caspase-3 (0.87-fold, 8.45-fold).
AN, thus, showed the potential beneficial effects on DM-induced male infertility, probably through its ability to counter DM induced apoptosis and inflammation in testicular tissues along with supporting anti-oxidant mechanisms. The extract could upregulate testosterone biosynthesis by modulating steroidogenic enzyme activity and stimulating FSH and LH levels. This action further supported the improvement of sperm functioning parameters in diabetic rats. These results provided the potential of AN as an alternative treatment for the clinical management of DM-impaired male reproductive function, offering hope for future research and clinical applications.
糖尿病（DM）通过调节细胞凋亡、氧化和炎症途径损害男性生殖功能。本研究旨在筛选由银背藤（Argyreia nervosa (Burm.Fil.) Bojer.）（旋花科）根制备的水醇提取物（AN）减轻糖尿病诱导的实验大鼠雄性生殖功能损害的能力。
通过联合给予高脂饮食和单次注射链脲佐菌素（30 mg/kg）诱导糖尿病状态。用较高剂量的银背藤提取物（ANH）治疗的糖尿病大鼠空腹血糖降低（0.52倍）、异常精子形态减少（0.50倍）、精子DNA碎片化指数降低（0.74倍），同时精液质量得到改善。ANH使睾丸睾酮增加（1.40倍）、促卵泡生成素（FSH）增加（1.91倍）、促黄体生成素（LH）增加（1.69倍），以及类固醇生成急性调节蛋白（StAR）的mRNA表达增加（1.75倍）、17β-羟基类固醇脱氢酶（17β-HSD）增加（1.77倍）、3β-羟基类固醇脱氢酶（3β-HSD）增加（1.06倍）。它降低了丙二醛（MDA）（0.72倍），提高了超氧化物歧化酶（SOD）（1.93倍）和谷胱甘肽（GSH）（2.13倍）。ANH还降低了晚期糖基化终末产物受体（RAGE）的mRNA/蛋白质水平（0.75倍，8.81倍）、肿瘤坏死因子-α（TNF-α）（0.79倍，2.11倍）、NOD样受体蛋白3（NLRP3）（0.68倍，8.15倍）和半胱天冬酶-3（Caspase-3）（0.87倍，8.45倍）。
因此，AN对糖尿病诱导的男性不育显示出潜在的有益作用，可能是通过其对抗糖尿病诱导的睾丸组织细胞凋亡和炎症以及支持抗氧化机制的能力。该提取物可通过调节类固醇生成酶活性和刺激FSH和LH水平来上调睾酮生物合成。这一作用进一步支持了糖尿病大鼠精子功能参数的改善。这些结果为AN作为糖尿病损害男性生殖功能临床管理的替代治疗方法提供了潜力，为未来的研究和临床应用带来了希望。
