Coutermarsh-Ott Sheryl
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, USA.
Methods Mol Biol. 2019;1960:237-247. doi: 10.1007/978-1-4939-9167-9_21.
Toxoplasma gondii is an intracellular, apicomplexan parasite of great importance in both human and animal health. Current research has identified a variety of important and necessary factors specific to the parasite that enable it to infect and persist in a wide array of mammalian hosts. However, in order to continue to build our understanding of T. gondii pathogenesis, the relevance of these parasite characteristics needs continued investigation in animal models. In the following chapter, we present a model of intraperitoneal infection of C57BL/6 mice with T. gondii tachyzoites that, in C57BL/6 mice, elicits a strong acute immune response. Moreover, we present methods for sampling and analyzing peritoneal and bronchoalveolar lavage fluids in order to assess localized and systemic immune reactions to the parasite.
刚地弓形虫是一种细胞内顶复门寄生虫，在人类和动物健康方面都极为重要。目前的研究已经确定了该寄生虫特有的多种重要且必要的因素，这些因素使其能够在多种哺乳动物宿主中感染并持续存在。然而，为了持续增进我们对刚地弓形虫发病机制的理解，这些寄生虫特征的相关性仍需在动物模型中继续研究。在接下来的章节中，我们展示了用刚地弓形虫速殖子对C57BL/6小鼠进行腹腔感染的模型，在C57BL/6小鼠中，该模型会引发强烈的急性免疫反应。此外，我们还介绍了采集和分析腹膜液及支气管肺泡灌洗液的方法，以便评估对该寄生虫的局部和全身免疫反应。
