Zapata-Sifuentes Mauricio, Quispe-Salcedo Angela, Watanabe Taisuke, Kawase Tomoyuki, Ohshima Hayato
Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
Division of Oral Bioengineering, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
Regen Ther. 2024 Sep 6;26:741-748. doi: 10.1016/j.reth.2024.08.019. eCollection 2024 Jun.
Osseointegration, the direct contact between an implant and bone, can be achieved by direct and/or indirect osteogenesis. Platelet-rich plasma accelerates tissue regeneration, wound healing, and osseointegration. This study aimed to analyze the effects of leukocyte and platelet-rich plasma (L-PRP) on direct and indirect osteogenesis after implant placement in a mouse maxilla.
Blood was collected from the tail vein of 4-8-week-old male ICR mice and L-PRP was obtained after double-spin cycle centrifugation. After the right upper first molars of 4-week-old ICR mice were extracted while under deep anesthesia, the alveolar sockets were prepared with a drill, and titanium implants blasted with hydroxyapatite/β-tricalcium phosphate were placed into the cavity filled with 1.5 μL of L-PRP. Samples were collected from the animals 3-28 days after implantation, and immunohistochemistry for osteopontin, Ki67 (cell proliferation marker), cathepsin-K (osteoclast marker), and osteonectin (osteoblast marker) was performed.
Cell proliferation was significantly higher in the L-PRP group than in the control group on postoperative days 3 and 5. The activities of osteoclast-lineage cells and osteoblasts increased significantly on day 5 in the L-PRP group, indicating that L-PRP evoked an active cellular response. Indirect osteogenesis was significantly higher on days 7, 14, and 28, and the osseointegration rate was significantly higher on day 28 in the L-PRP group compared with the control group.
L-PRP enhances osseointegration by promoting mesenchymal cell proliferation, osteoclastic and osteoblastic activities, and indirect osteogenesis.
骨结合，即种植体与骨之间的直接接触，可通过直接和/或间接成骨实现。富血小板血浆可加速组织再生、伤口愈合和骨结合。本研究旨在分析富含白细胞和血小板的血浆（L-PRP）对小鼠上颌植入种植体后直接和间接成骨的影响。
从4-8周龄雄性ICR小鼠的尾静脉采集血液，经双循环离心后获得L-PRP。在深度麻醉下拔除4周龄ICR小鼠的右上第一磨牙后，用钻头制备牙槽窝，将用羟基磷灰石/β-磷酸三钙喷砂处理的钛种植体植入充满1.5 μL L-PRP的腔内。在植入后3-28天从动物身上采集样本，并进行骨桥蛋白、Ki67（细胞增殖标志物）、组织蛋白酶K（破骨细胞标志物）和骨连接蛋白（成骨细胞标志物）的免疫组织化学检测。
术后第3天和第5天，L-PRP组的细胞增殖明显高于对照组。L-PRP组在第5天破骨细胞系细胞和成骨细胞的活性显著增加，表明L-PRP引发了活跃的细胞反应。与对照组相比，L-PRP组在第7天、第14天和第28天的间接成骨明显更高，在第28天的骨结合率也明显更高。
L-PRP通过促进间充质细胞增殖、破骨细胞和成骨细胞活性以及间接成骨来增强骨结合。
