PET 显像肝细胞癌：F-氟丙酸作为 F-氟脱氧葡萄糖的补充放射性示踪剂。
PET Imaging of Hepatocellular Carcinomas: F-Fluoropropionic Acid as a Complementary Radiotracer for F-Fluorodeoxyglucose.
机构信息
1 Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangzhou, China.
2 Department of Nuclear Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
出版信息
Mol Imaging. 2019 Jan-Dec;18:1536012118821032. doi: 10.1177/1536012118821032.
OBJECTIVE
To evaluate the preclinical value of F-fluoropropionic acid (F-FPA) and F-fluorodeoxyglucose (F-FDG) positron emission tomography (PET) for imaging HCCs.
METHODS
The F-FPA and F-FDG uptake patterns in 3 HCC cell lines (Hep3B, HepG2, and SK-Hep1) were assessed in vitro and in vivo. The F-FPA uptake mechanism was investigated using inhibition experiments with orlistat and 5-tetradecyloxy-2-furoic acid. The F-FPA PET imaging was performed in different tumor animal models and compared with F-FDG. We also evaluated the expressions of glucose transporter-1 (GLUT1), fatty acid synthase (FASN), and matrix metalloproteinase-2 (MMP2) in these cell lines.
RESULTS
In vitro experiments showed that the radiotracer uptake patterns were complementary in the HCC cell lines. Orlistat and 5-tetradecyloxy-2-furoic acid decreased the uptake of F-FPA. The tumor-to-liver ratio of F-FPA was superior to that of F-FDG in the SK-Hep1 and HepG2 tumors ( P < .05). However, in the Hep3B tumors, the tumor-to-liver normalized uptake of F-FDG was higher than F-FPA ( P < .01). FASN was highly expressed in cell lines with high F-FPA uptake, whereas GLUT1 was highly expressed in cell lines with high F-FDG uptake. The F-FPA uptake correlated with FASN ( r = 0.89, P = .014) and MMP2 ( r = 0.77, P = .002) expressions.
CONCLUSIONS
PET imaging with F-FPA combined with F-FDG can be an alternative for detecting HCC.
目的
评估 F-氟丙酸（F-FPA）和 F-氟脱氧葡萄糖（F-FDG）正电子发射断层扫描（PET）在 HCC 成像中的临床前价值。
方法
在体外和体内评估了 3 种 HCC 细胞系（Hep3B、HepG2 和 SK-Hep1）中 F-FPA 和 F-FDG 的摄取模式。通过使用奥利司他和 5-十四烷氧基-2-糠酸进行抑制实验，研究了 F-FPA 摄取机制。在不同的肿瘤动物模型中进行了 F-FPA PET 成像，并与 F-FDG 进行了比较。我们还评估了这些细胞系中葡萄糖转运蛋白 1（GLUT1）、脂肪酸合酶（FASN）和基质金属蛋白酶 2（MMP2）的表达。
结果
体外实验表明，示踪剂在 HCC 细胞系中的摄取模式是互补的。奥利司他和 5-十四烷氧基-2-糠酸降低了 F-FPA 的摄取。F-FPA 的肿瘤与肝脏比值在 SK-Hep1 和 HepG2 肿瘤中优于 F-FDG（P<0.05）。然而，在 Hep3B 肿瘤中，F-FDG 的肿瘤与肝脏归一化摄取高于 F-FPA（P<0.01）。FASN 在 F-FPA 摄取较高的细胞系中高度表达，而 GLUT1 在 F-FDG 摄取较高的细胞系中高度表达。F-FPA 的摄取与 FASN（r=0.89，P=0.014）和 MMP2（r=0.77，P=0.002）的表达相关。
结论
F-FPA 与 F-FDG 联合 PET 成像可以作为检测 HCC 的替代方法。
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