Kakkava Eirini, Romito Marilisa, Conkey Donald B, Loterie Damien, Stankovic Konstantina M, Moser Christophe, Psaltis Demetri
Optics Laboratory, School of Engineering, École Polytechnique Fédérale De Lausanne, Lausanne, Switzerland.
Laboratory of Applied Photonic Devices, School of Engineering, École Polytechnique Fédérale De Lausanne, Lausanne, Switzerland.
Biomed Opt Express. 2019 Jan 7;10(2):423-433. doi: 10.1364/BOE.10.000423. eCollection 2019 Feb 1.
We demonstrate the ability of a multimode fiber probe to provide two-photon fluorescence (TPF) imaging feedback that guides the femtosecond laser ablation (FLA) in biological samples for highly selective modifications. We implement the system through the propagation of high power femtosecond pulses through a graded-index (GRIN) multimode fiber and we investigate the limitations posed by the high laser peak intensities required for laser ablation. We demonstrate that the GRIN fiber probe can deliver laser intensities up to 1.5x10 W/cm, sufficient for the ablation of a wide range of materials, including biological samples. Wavefront shaping through an ultrathin probe of around 400 μm in diameter is used for diffraction limited focusing and digital scanning of the focus spot. Selective FLA of cochlear hair cells is performed based on the TPF images obtained through the same multimode fiber probe.
我们展示了一种多模光纤探头提供双光子荧光（TPF）成像反馈的能力，该反馈可指导生物样品中的飞秒激光烧蚀（FLA），以实现高度选择性的修饰。我们通过高功率飞秒脉冲在渐变折射率（GRIN）多模光纤中的传播来实现该系统，并研究激光烧蚀所需的高激光峰值强度所带来的限制。我们证明，GRIN光纤探头可提供高达1.5x10 W/cm的激光强度，足以烧蚀包括生物样品在内的多种材料。通过直径约400μm的超薄探头进行波前整形，用于衍射极限聚焦和焦点光斑的数字扫描。基于通过同一多模光纤探头获得的TPF图像，对耳蜗毛细胞进行选择性FLA。
