Broeckx Sarah Y, Pille Frederik, Buntinx Simon, Van Brantegem Leen, Duchateau Luc, Oosterlinck Maarten, Chiers Koen, Bertone Alicia L, Spaas Jan H, Martens Ann M
Am J Vet Res. 2019 Mar;80(3):246-258. doi: 10.2460/ajvr.80.3.246.
OBJECTIVE To evaluate lameness and morphological changes associated with an osteochondral fragment-groove procedure as a means of experimental induction of metacarpophalangeal (MCP) joint osteoarthritis within an 11-week period in horses. ANIMALS 6 nonlame adult warmbloods. PROCEDURES The right MCP joint of each horse underwent an osteochondral fragment-groove procedure (day 0). After 1 week of stall rest (ie, starting day 7), each horse was trained daily on a treadmill. Weekly, horses underwent visual and inertial sensor-based assessments of lameness. Both MCP joints were assessed radiographically on days 0 (before surgery), 1, 35, and 77. A synovial fluid sample was collected from the right MCP joint on days 0 (before surgery), 35, 36, 49, 63, and 77 for cytologic and biomarker analyses. On day 77, each horse was euthanized; both MCP joints were evaluated macroscopically and histologically. RESULTS Right forelimb lameness was detected visually and by the inertial sensor system when horses were moving on a straight line after distal forelimb flexion or circling left on days 14 to 77. Compared with presurgical values, synovial fluid interleukin-6, prostaglandin E2, hyaluronic acid, and interleukin-1 receptor antagonist protein concentrations were increased at 2 or 3 time points, whereas tumor necrosis factor-α and interleukin-10 concentrations were decreased at 1 time point. Gross examination of all right MCP joints revealed synovitis and wear lines; synovitis was confirmed histologically. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that a combined osteochondral fragment-groove procedure can be used to induce clinically and grossly observable early MCP joint osteoarthritis during an 11-week period in horses.
目的 评估骨软骨碎片-凹槽手术作为在11周内实验性诱导马掌指关节（MCP）骨关节炎的一种方法所导致的跛行及形态学变化。动物 6匹无跛行的成年温血马。方法 每匹马的右MCP关节接受骨软骨碎片-凹槽手术（第0天）。在厩内休息1周后（即从第7天开始），每匹马每天在跑步机上训练。每周对马进行基于视觉和惯性传感器的跛行评估。在第0天（手术前）、1天、35天和77天对两个MCP关节进行放射学评估。在第0天（手术前）、35天、36天、49天、63天和77天从右MCP关节采集滑液样本进行细胞学和生物标志物分析。在第77天，对每匹马实施安乐死；对两个MCP关节进行大体和组织学评估。结果 当马在第14至77天进行前肢远端屈曲后直线行走或向左转圈时，通过视觉和惯性传感器系统检测到右前肢跛行。与术前值相比，滑液白细胞介素-6、前列腺素E2、透明质酸和白细胞介素-1受体拮抗剂蛋白浓度在2或3个时间点升高，而肿瘤坏死因子-α和白细胞介素-10浓度在1个时间点降低。对所有右MCP关节的大体检查显示滑膜炎和磨损线；组织学证实为滑膜炎。结论及临床意义 结果表明，骨软骨碎片-凹槽联合手术可用于在11周内诱导马出现临床和大体可观察到的早期MCP关节骨关节炎。
