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人初乳中存在的抗大肠杆菌γ-A抗体的血清学特性。

Serological properties of gamma-A antibodies to Escherichia coli present in human colostrum.

作者信息

Adinolfi M, Glynn A A, Lindsay M, Milne C M

出版信息

Immunology. 1966 Jun;10(6):517-26.

PMID:5330427
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1423745/
Abstract

Antibodies against WF 96 and WF 61 present in human colostrum and serum were fractionated by DEAE-cellulose chromatography. Using the haemagglutination test it was found that the antibodies present in colostrum were recovered in the fraction containing the bulk of γA-globulin, whereas the antibodies present in serum were recovered in the fraction containing the bulk of γM-globulin. In the presence of human or guinea-pig complement the antibodies present in colostrum did not lyse red cells coated with bacterial polysaccharides whereas the antibodies present in serum were lytic. When the properties of γA and γM antibodies were studied using a bacteriolytic system, it was observed that γA-globulin lysed bacteria only in the presence of both complement and lysozyme; in this respect γAbacterial antibodies behaved differently from γM antibodies which were bacteriolytic in the presence of complement alone, without lysozyme. The effect of treating γA and γM antibodies with 2-mercaptoethanol at neutral pH and of heating at 56° was investigated.

摘要

采用DEAE - 纤维素色谱法对人初乳和血清中存在的抗WF 96和WF 61抗体进行分离。通过血凝试验发现，初乳中存在的抗体在含有大部分γA球蛋白的组分中回收，而血清中存在的抗体在含有大部分γM球蛋白的组分中回收。在人或豚鼠补体存在的情况下，初乳中存在的抗体不会溶解包被有细菌多糖的红细胞，而血清中存在的抗体具有溶细胞作用。当使用溶菌系统研究γA和γM抗体的特性时，观察到γA球蛋白仅在补体和溶菌酶同时存在时才溶解细菌；在这方面，γA细菌抗体的行为与γM抗体不同，γM抗体仅在补体存在而无溶菌酶时具有溶菌作用。研究了在中性pH下用2 - 巯基乙醇处理γA和γM抗体以及在56℃加热的效果。

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