Zhao Guohua, Bae Jung Yoon, Zheng Zhenlong, Park Hae Seok, Chung Kee Yang, Roh Mi Ryung, Jin Zhehu
Department of Dermatology, Yanbian University Hospital, Yanji, P.R. China.
Department of Pharmacology, Yonsei University College of Medicine, Seoul, Republic of Korea.
Anticancer Res. 2019 Apr;39(4):1849-1857. doi: 10.21873/anticanres.13292.
BACKGROUND/AIM: Melanoma-associated antigen A12 (MAGEA12) has recently been reported as a repressor of tumor-suppressor genes. This study aimed to investigate the implications of MAGEA12 expression in the pathogenesis of cutaneous squamous cell carcinoma (cSCC).
MAGEA12 and p21 expression were investigated in 15 samples of normal skin and 111 of cSCC tissues by immunohistochemistry. The biological functions of MAGEA12 in cSCC were also investigated both in vitro and in vivo.
Expression of both MAGEA12 and p21 was significantly increased in cSCC. MAGEA12 expression showed a positive correlation, while p21 expression showed negative correlation with the recurrence-free survival of patients with cSCC. In addition, MAGEA12 knockdown significantly attenuated proliferative, migratory, invasive, and tumorigenic activities of cSCC cells and was negatively correlated with p21 expression both in vitro and in vivo.
MAGEA12-mediated down-regulation of p21 may be involved in cSCC pathogenesis and MAGEA12 may serve as a molecular biomarker in cSCC.
背景/目的：黑色素瘤相关抗原A12（MAGEA12）最近被报道为肿瘤抑制基因的一种抑制因子。本研究旨在探讨MAGEA12表达在皮肤鳞状细胞癌（cSCC）发病机制中的意义。
采用免疫组织化学方法检测15例正常皮肤样本和111例cSCC组织样本中MAGEA12和p21的表达。还在体外和体内研究了MAGEA12在cSCC中的生物学功能。
cSCC中MAGEA12和p21的表达均显著增加。MAGEA12表达与cSCC患者的无复发生存呈正相关，而p21表达与cSCC患者的无复发生存呈负相关。此外，MAGEA12基因敲低显著减弱了cSCC细胞的增殖、迁移、侵袭和致瘤活性，且在体外和体内均与p21表达呈负相关。
MAGEA12介导的p21下调可能参与cSCC的发病机制，且MAGEA12可能作为cSCC的一种分子生物标志物。
