Su Qing, Yang Jing, Fu Qing Yun, Jia Fei Yun, Li Suo Ping, Li Yong, Li You Yong
Henan Institute of Science and Technology/Collaborative Innovation Center of Modern Biological Breeding, Xinxiang, 453003 China.
2Henan University, Kaifeng, 475000 China.
Physiol Mol Biol Plants. 2019 Jan;25(1):263-275. doi: 10.1007/s12298-018-0626-0. Epub 2018 Nov 28.
Bainong male sterile (BNS) wheat ( L.) is a thermo-sensitive genic male sterile line with excellent sterility and self-restoration. We focused on transcriptional profiles of differentially expressed probes between BNS sterile and fertile anthers. Anthers, rachis and spikes from sterile line and fertile line were collected. Extracted RNA was assayed using wheat expression microarray and Gene Ontology was analyzed using Cytoscape with ClueGO. An indole (indole-3-acetic acid: IAA) metabolism pathway sub-network was almost formed in all differentially expressed profiles between sterile and fertile samples. IAA sub-network contained four nodes of indole and alkaloid metabolism connecting main network via indole compounds. This sub-network was absent in rachis and intact in transformed fertile anthers, which was the main differently expressed metabolism pathway in F anthers with restorer genes. Alkaloid metabolism was absent in sterile anthers. Abnormal metabolism of IAA may be involved in BNS sterility. BNS transformation may be regulated by the production of IAA and alkaloid metabolism pathway, which favor the safe utilization of the sterile line in hybrid wheat production.
百农雄性不育（BNS）小麦是一种具有优良不育性和自我恢复能力的温敏核雄性不育系。我们重点研究了BNS不育花药和可育花药之间差异表达探针的转录谱。收集了不育系和可育系的花药、穗轴和小穗。提取的RNA用小麦表达微阵列进行检测，基因本体论用带有ClueGO的Cytoscape进行分析。在不育和可育样本之间的所有差异表达谱中，几乎都形成了一个吲哚（吲哚 - 3 - 乙酸：IAA）代谢途径子网络。IAA子网络包含四个吲哚和生物碱代谢节点，通过吲哚化合物连接主网络。该子网络在穗轴中不存在，在转化的可育花药中完整，这是具有恢复基因的F花药中主要的差异表达代谢途径。不育花药中不存在生物碱代谢。IAA的异常代谢可能与BNS不育有关。BNS的转化可能受IAA的产生和生物碱代谢途径的调控，这有利于不育系在杂交小麦生产中的安全利用。
