Isnaini Isnaini, Yasmina Alfi, Nur’amin Hendra Wana
Department of Pharmacology and Therapeutics, Faculty of Medicine, Lambung Mangkurat University, South Kalimantan, Indonesia. Email:
Asian Pac J Cancer Prev. 2019 Feb 26;20(2):639-643. doi: 10.31557/APJCP.2019.20.2.639.
Melastoma malabathricum L. is a type of plant naturally grows in Kalimantan that has medicinal properties. Ethanolic extract of M. malabathricum L. flower has quercetin and kaempferol contents that have antioxidant and anticancer activities. But the antioxidant and cytotoxicity activities of M. malabathricum L. fruit ethanolic extract were not known. This study measured the quercetin and kaempferol level in M. malabathricum L. fruit ethanolic extract using HPLC MS/MS, antioxidant activity using DPPH method, and cytotoxicity activity using Brine Shrimp Lethality Test (BSLT) method. Results showed the level of quercetin and kaempferol from M. malabathricum L fruit ethanolic extract are 67.78 μg/g and 43.52 μg/g, respectively. Beside, the antioxidant activity with by IC50 was 16.82±0.24 ppm and 7.38±0.41 ppm. The cytotoxicity activities of M. malabathricum L. fruit ethanolic extract and quercetin are shown by the LC50 of 313.44 ppm (95%CI 283.97-344.43) and 37.24 ppm, respectively.
野牡丹是一种自然生长在加里曼丹的植物，具有药用特性。野牡丹花的乙醇提取物含有具有抗氧化和抗癌活性的槲皮素和山奈酚。但野牡丹果实乙醇提取物的抗氧化和细胞毒性活性尚不清楚。本研究采用高效液相色谱-质谱/质谱法测定野牡丹果实乙醇提取物中槲皮素和山奈酚的含量，采用DPPH法测定抗氧化活性，采用卤虫致死试验（BSLT）法测定细胞毒性活性。结果表明，野牡丹果实乙醇提取物中槲皮素和山奈酚的含量分别为67.78μg/g和43.52μg/g。此外，其抗氧化活性的IC50分别为16.82±0.24ppm和7.38±0.41ppm。野牡丹果实乙醇提取物和槲皮素的细胞毒性活性分别以LC50为313.44ppm（95%CI 283.97-344.43）和37.24ppm表示。
