探讨上颌窦后鼻孔息肉中人乳头瘤病毒（HPV）和 Epstein-Barr 病毒（EBV）的感染情况。

Investigation of Human Papillomavirus (HPV) and Epstein-Barr Virus (EBV) in antrochoanal polyps.

机构信息

Department of Otorhinolaryngology, Dr. Ali Kemal Belviranli Maternity and Children's Hospital, Turkey.

Department of Otorhinolaryngology, Medical Sciences University Konya Training and Research Hospital, Turkey.

出版信息

Am J Otolaryngol. 2019 May-Jun;40(3):389-392. doi: 10.1016/j.amjoto.2019.02.008. Epub 2019 Feb 19.

DOI:10.1016/j.amjoto.2019.02.008

PMID:30808528

Abstract

OBJECTIVES/HYPOTHESES: This study aimed to investigate the presence of HPV (HPV types 11 and 16) and EBV in antrochoanal polyps and to contribute to the current literature in this regard.

STUDY DESIGN

A case-control study.

METHODS

A total of 100 patients (including 43 patients undergoing surgery for antrochoanal polyp, 27 patients undergoing surgery for nasal polyp, and 30 patients undergoing surgery for hypertrophic inferior turbinate) were included in this study. DNA was isolated from formalin-fixed, paraffin-embedded samples with the aid of the Bioneer's AccuPrep Genomic DNA Extraction Kit. In the obtained genomic DNAs, while the detection of HPV DNA was performed using the nested-PCR method, the detection of HPV types 11/16 and EBV DNA was performed using the RT-PCR method.

RESULTS

The mean age of the patients with antrochoanal polyp was 26.7 ± 15.4 years (range 7-70). There were 20 (46.5%) women and 23 (53.5%) men in the antrochoanal polyp group. HPV DNA was positively detected using the nested-PCR method in 14 (32.6%) of the patients with antrochoanal polyp and in 3 (11.1%) of the patients with nasal polyp. HPV DNA was not detected in the hypertrophic inferior turbinate group (control group). There was a statistically significant difference between all groups in terms of HPV DNA positivity. In the antrochoanal polyp group, 2 patients had HPV 11 positivity and 12 patients had HPV 16 positivity. In the nasal polyp group, 1 patient had HPV 11 positivity and 2 patients had HPV 16 positivity. EBV DNA was positively detected in 16 (37.2%) of the patients with antrochoanal polyp, in 11 (40.7%) of the patients with nasal polyp and in 8 (26.7%) of the patients with hypertrophic inferior turbinate, respectively. There was no statistically significant difference between the groups in terms of EBV DNA positivity.

CONCLUSIONS

This study demonstrates that there is a need for further studies investigating the presence of viruses in antrochoanal polyps.

摘要

目的/假设：本研究旨在调查 HPV（HPV 型 11 和 16）和 EBV 在antrochoanal 息肉中的存在情况，并为这方面的现有文献做出贡献。

研究设计

病例对照研究。

方法

本研究共纳入 100 例患者（包括 43 例行 antralchoanal 息肉切除术的患者、27 例行鼻息肉切除术的患者和 30 例行鼻甲肥大切除术的患者）。使用 Bioneer 的 AccuPrep 基因组 DNA 提取试剂盒从福尔马林固定、石蜡包埋的样本中分离 DNA。在获得的基因组 DNA 中，使用巢式 PCR 方法检测 HPV DNA，使用 RT-PCR 方法检测 HPV 型 11/16 和 EBV DNA。

结果

antrochoanal 息肉患者的平均年龄为 26.7±15.4 岁（7-70 岁）。antrochoanal 息肉组中，女性 20 例（46.5%），男性 23 例（53.5%）。14 例（32.6%）antrochoanal 息肉患者和 3 例（11.1%）鼻息肉患者经巢式 PCR 方法检测到 HPV DNA 阳性。鼻甲肥大组（对照组）未检测到 HPV DNA。所有组之间 HPV DNA 阳性率存在统计学显著差异。在 antralchoanal 息肉组中，2 例患者 HPV11 阳性，12 例患者 HPV16 阳性。在鼻息肉组中，1 例患者 HPV11 阳性，2 例患者 HPV16 阳性。16 例（37.2%）antrochoanal 息肉患者、11 例（40.7%）鼻息肉患者和 8 例（26.7%）鼻甲肥大患者经 EBV DNA 检测呈阳性。各组 EBV DNA 阳性率无统计学显著差异。